Dielectrophoretic detection and quantification of hybridized DNA molecules on nano-genetic particles
Dielectrophoretic detection and quantification of hybridized DNA molecules on nano-genetic particles
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DOI：
10.1002/elps.200800528
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发表时间：
2008-12-01
期刊：
影响因子：
2.9
通讯作者：
Chang, Hsueh-Chia
中科院分区：
文献类型：
--
作者：
Gagnon, Zachary;Senapati, Satyajyoti;Chang, Hsueh-Chia
DNA-DNA杂交反应100 nm的谷胱甘肽功能化的二氧化硅纳米粒子被发现敏感地影响的幅度和方向的介电泳迁移率的粒子在纳摩尔目标ssDNA浓度。这样的灵敏度允许在没有荧光标记和共聚焦显微镜的情况下通过在四极电极阵列上成像par-tide悬浮液的交叉频率（cof）来视觉检测杂交事件。与有效粒子半径和Zeta电位测量的强相关性表明，介电泳COF不仅提供了成功的功能化和杂交的敏感签名，而且还提供了三种不同的DNA表面构象，出现在不同的表面密度的杂交DNA。正确标准化的cof校准图允许简化目标ssDNA浓度的定量。这些结果提供了一个简单，快速和便携式的基因检测方法，适用于实验室外使用。
DNA-DNA hybridization reactions on 100 nm oligonucleotide-functionalized silica nanoparticles are found to sensitively affect the amplitude and direction of the dielectrophoretic mobility of the particles at nanomolar target ssDNA concentrations. Such sensitivity permits visual detection of the hybridization event without fluorescent labeling and confocal microscopy by imaging the cross-over frequency (cof) of the par-tide suspension on a quadrupole electrode array. Strong correlation with effective particle radius and zeta-potential measurements suggests that the dielectrophoretic cof offers not just sensitive signatures for successful functionalization and hybridization but also those for three distinct DNA surface conformations that appear at different surface densities of hybridized DNA. A properly normalized cof calibration chart allows simplified quantification of the target ssDNA concentrations. These results provide a simple, rapid and portable genetic detection method compatible for use outside the laboratory.