Oxidative stress is involved in Patulin induced apoptosis in HEK293 cells
Oxidative stress is involved in Patulin induced apoptosis in HEK293 cells
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氧化应激参与棒曲霉素诱导 HEK293 细胞凋亡
DOI：
10.1016/j.toxicon.2014.12.002
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发表时间：
2015-02-01
期刊：
影响因子：
2.8
通讯作者：
Wang, Qian
中科院分区：
文献类型：
--
作者：
Zhang, Baigang;Peng, Xiaoli;Wang, Qian
棒曲霉素（PAT）是在农产品中发现的最广泛传播的真菌毒素之一，可引起肾损伤。然而，细胞损伤的机制仍然不清楚。在这项研究中，用PAT（2.5-15 μ M）处理人胚肾细胞（HEK 293）。MTT法检测细胞毒性，流式细胞仪检测凋亡细胞，Hoechst 33342荧光显微镜下染色质浓缩和核碎裂法鉴定凋亡细胞。用DCFH-DA荧光法测定活性氧（ROS）。通过测定超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、过氧化氢酶（CAT）活性及丙二醛（MDA）含量，探讨PAT诱导细胞凋亡的可能机制。JC-1探针测定线粒体膜电位。结果表明，PAT可剂量依赖性地抑制HEK 293细胞的生长，并导致HEK 293细胞凋亡。PAT处理可诱导ROS和MDA的积累，同时伴有SOD、CAT、GSH的损失和线粒体膜电位的破坏。这些数据表明，PAT可以诱导HEK 293细胞凋亡，其中涉及氧化应激。(C)2014爱思唯尔有限公司版权所有。
Patulin (PAT) is one of the most widely disseminated mycotoxins found in agricultural products and could cause renal damage. However, the mechanism of cell damage remains obscure. In this study, the human embryonic kidney cells (HEK293) were treated with PAT (2.5-15 mu M). The cytotoxicity was assessed with MTT assay and apoptotic cells were detected by flow cytometry, and further identified by chromatin condensation and nuclear fragmentation with Hoechst 33342 under fluorescence microscope. Reactive oxygen species (ROS) with DCFH-DA was analyzed by fluorometry. The activities of superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT) and malondialdehyde (MDA) content were determined to reveal the potential mechanism of PAT induced apoptosis. The mitochondrial membrane potential was measured by JC-1 probe. The results showed that PAT dose-dependently inhibited the growth of HEK293 cells and resulted in apoptosis in HEK293 cells. Treatment with PAT could induce ROS and MDA accumulation, accompanied by the losses of SOD, CAT, GSH and disruption of the mitochondrial membrane potential. These data suggest that PAT may induce apoptosis in HEK293 cells, in which oxidative stress is involved. (C) 2014 Elsevier Ltd. All rights reserved.