Evolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates
Evolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates
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DOI：
10.1016/j.ydbio.2003.12.016
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发表时间：
2004-04-01
影响因子：
2.7
通讯作者：
Argenton, F
中科院分区：
文献类型：
--
作者：
Zecchin, E;Mavropoulos, A;Argenton, F
我们对斑马鱼 ptf1a 基因进行了表征和作图，分析了其胚胎表达，并研究了其在胰腺发育中的作用。原位杂交实验表明，从12体节期到48 hpf，ptf1a在斑马鱼胚胎的脊髓、后脑、小脑、视网膜和胰腺中动态表达。在内胚层中，ptf1a 最初于 32 hpf 时在 pdx1 表达域的腹侧部分表达； ptf1a 在位于胚胎左侧肝原基后方和由后背胰原基产生的内分泌岛前方的细胞亚群中表达。然后 ptf1a 表达域芽产生前腹侧胰腺原基，该原基向后生长，最终吞没内分泌胰岛。到 72 hpf，ptf1a 继续在源自前腹原基的外分泌室中表达。吗啡啉诱导的 ptf1a 功能丧失会抑制外分泌标记物的表达，而胰岛中的内分泌标记物不受影响。在智力炸弹 (mib) 突变体中，δ 介导的缺口信号传导存在缺陷 [Dev. Cell 4 (2003) 67]，ptf1a 正常表达。此外，慢肌省略（smu）突变体由于刺猬信号传导缺陷而缺乏内分泌标记的表达[Curr。生物。 11(2001) 1358]表现出正常水平的ptf1a。这表明刺猬信号在前腹侧（主要是外分泌）和后背侧（内分泌）胰腺原基的规范中发挥着不同的遗传作用。 (C) 2004 Elsevier Inc. 保留所有权利。
We have characterized and mapped the zebrafish ptf1a gene, analyzed its embryonic expression, and studied its role in pancreas development. In situ hybridization experiments show th at from the 12-somite stage to 48 hpf, ptf1a is dynamically expressed in the spinal cord, hindbrain, cerebellum, retina, and pancreas of zebrafish embryos. Within the endoderm, ptf1a is initially expressed at 32 hpf in the ventral portion of the pdx1 expression domain; ptf1a is expressed in a subset of cells located on the left side of the embryo posteriorly to the liver primordium and anteriorly to the endocrine islet that arises from the posterodorsal pancreatic anlage. Then the ptf1a expression domain buds giving rise to the anteroventral pancreatic anlage that grows posteriorly to eventually engulf the endocrine islet. By 72 hpf, ptf1a continues to be expressed in the exocrine compartment derived from the anteroventral anlage. Morpholino-induced ptf1a loss of function suppresses the expression of the exocrine markers, while the endocrine markers in the islet are unaffected. In mind bomb (mib) mutants, in which delta-mediated notch signalling is defective [Dev. Cell 4 (2003) 67], ptf1a is normally expressed. In addition, the slow-muscle-omitted (smu) mutants that lack expression of endocrine markers because of a defective hedgehog signalling [Curr. Biol. 11(2001) 1358] exhibit normal levels of ptf1a. This indicates that hedgehog signaling plays a different genetic role in the specification of the anteroventral (mostly exocrine) and posterodorsal (endocrine) pancreatic anlagen. (C) 2004 Elsevier Inc. All rights reserved.