ALTERED FRUIT RIPENING AND LEAF SENESCENCE IN TOMATOES EXPRESSING AN ANTISENSE ETHYLENE-FORMING ENZYME TRANSGENE
ALTERED FRUIT RIPENING AND LEAF SENESCENCE IN TOMATOES EXPRESSING AN ANTISENSE ETHYLENE-FORMING ENZYME TRANSGENE
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DOI：
10.1111/j.1365-313x.1993.tb00167.x
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发表时间：
1993-03-01
期刊：
影响因子：
7.2
通讯作者：
GRIERSON, D
中科院分区：
文献类型：
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作者：
PICTON, S;BARTON, SL;GRIERSON, D
利用反义基因抑制乙烯形成酶（EFE）合成的番茄植株，在生化和分子水平上研究乙烯在果实成熟中的作用。在植物上和植物外成熟的 EFE 反义果实中观察到总番茄红素积累减少。 EFE 反义水果的过熟率和水果腐败率也有所降低。成熟的抑制程度取决于果实与植物分离的发育阶段。当果实在颜色变化开始之前从葡萄藤上分离时，对 EFE 反义果实成熟的影响更加明显，并且与与许多先前表征的成熟相关 cDNA 同源的 mRNA 积累水平的变化有关。乙烯处理可以部分恢复分离的 EFE-反义果实的延迟成熟。除了在成熟果实中观察到的变化外，在 EFE 反义植物中还观察到叶子衰老开始的时间延迟，这表明 EFE 反义基因的生理效应以及相关的乙烯进化减少并不局限于果实成熟。
Tomato plants in which the synthesis of ethylene-forming enzyme (EFE) had been inhibited by an anti-sense gene were used to study the role of ethylene in fruit ripening at the biochemical and molecular level. A reduction in total lycopene accumulation was observed in EFE-antisense fruit ripened both on and off the plant. The rate of over-ripening and fruit spoilage was also reduced in the EFE-antisense fruit. The degree of inhibition of ripening was shown to be dependent upon the stage of development at which the fruit were detached from the plant. The effects on ripening in EFE-antisense fruit were much more pronounced when fruit were detached from the vine before the onset of colour change, and were associated with changes in the level of accumulation of mRNAs homologous to a number of previously characterized ripening-related cDNAs. The retarded ripening of detached EFE-antisense fruit could be partially restored by ethylene treatment. In addition to changes observed in ripening fruit, a temporal delay in the onset of foliar senescence was observed in EFE-antisense plants, indicating that the physiological effects of the EFE-antisense gene, and the associated reduction in ethylene evolution, are not confined specifically to fruit ripening.