Subarachnoid hemorrhage induces dynamic changes in regional cerebral metabolism in rats.
Subarachnoid hemorrhage induces dynamic changes in regional cerebral metabolism in rats.
复制标题
蛛网膜下腔出血引起大鼠局部脑代谢的动态变化。
DOI：
10.1089/08977150252932406
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Hovda,DavidA
中科院分区：
文献类型：
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作者：
Glenn,ThomasC;Patel,AmanB;Martin,NeilA;Samii,Amir;DeJesus,Catherine;Hovda,DavidA
在蛛网膜下腔出血(SAH)后，成年大鼠表现出动态的区域性脑糖代谢变化，其特征是代谢率增加，随后细胞色素氧化酶(CO)上调。我们评估了23个大脑感兴趣区(ROI)的局部脑葡萄糖代谢率(lCMRglc：(Mol/100 g/min))和CO代谢率。假手术组动物接受麻醉和表面手术；生理盐水对照组大鼠枕大池注射0.9%生理盐水；蛛网膜下腔出血大鼠枕大池注射自体血。这些血液，通过用放射性碳14标记的白蛋白来测量，分布在整个大脑中，但以腹壁为主。分别于注射后0d(3h)、1d、3d和7d处死动物，用[14C]2-脱氧-D-葡萄糖放射自显影和CO组织化学方法分析ROI。SAH大鼠的lCMRglc在多个区域升高(比假手术高0.7%~32.2%)。细胞色素氧化酶也比假手术组高1%~9%，在第3天达到高峰。相反，生理盐水对照组从第0天到第7天表现出对lCMRglc(比假手术水平低11%~35%)和CO(比假手术水平低4%~11%)的持续抑制。在所有时间点，生理盐水对照组的ROI都表现出这种代谢抑制，91%到95%的生理盐水对照ROI表现出比假手术低的CO水平。总体而言，SAH组的lCMRglc和CO水平高于生理盐水对照组ROI组。然而，当考虑蛛网膜下腔血对SAH动物代谢变化的影响时，CO和2DG水平与14C-白蛋白结合量没有很好的相关性。虽然之前的研究在SAH后不久就测量了葡萄糖和一氧化碳的代谢率，但这是第一次在同一SAH大鼠模型中同时进行这些测量。
Following a subarachnoid hemorrhage (SAH), adult rats exhibit dynamic regional changes in cerebral glucose metabolism characterized by an increase in metabolic rates and a subsequent upregulation of cytochrome oxidase (CO). We evaluated both local cerebral metabolic rates for glucose (lCMRglc: (mol/100 g/min) and CO in 23 brain regions of interest (ROI). Sham animals underwent anesthesia and superficial surgery; saline-controls received an injection of 0.9% saline into the cisterna magna; and SAH rats received an injection of autologous blood into the cisterna magna. This blood, measured by albumin labeled with radioactive carbon 14, distributed throughout the brain but predominated ventrally. After experimental animals were sacrificed at day 0 (3 h), 1, 3, and 7 days postinjection, ROI were analyzed using [14C]2-deoxy-D-glucose autoradiography and CO histochemistry. lCMRglc in SAH rats increased in many regions (ranging from 0.7% to 32.2% above sham levels). Cytochrome oxidase also increased from 1% to 9% above sham levels, peaking on day 3. Conversely, saline-controls exhibited prolonged depression of lCMRglc (ranging from 11% to 35% below sham levels) and CO (ranging from 4% to 11% below sham levels) from day 0 through day 7. All saline-control ROI for all time points showed this metabolic depression, and between 91% and 95% of saline-control ROI presented lower CO levels as compared to sham. Overall, lCMRglc and CO levels were greater in SAH than in saline-control ROI. However, when considering the influence of subarachnoid blood on metabolic changes in SAH animals, both CO and 2DG levels did not correlate well with the amount of14C-albumin binding. While previous studies have measured both metabolic rates of glucose and CO soon after SAH, this is the first to simultaneously conduct these measurements in the same SAH rat model.