Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type.
Genotyping of Turkish environmental Cryptococcus neoformans var. neoformans isolates by pulsed field gel electrophoresis and mating type.
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土耳其环境新型隐球菌变种的基因分型
DOI：
10.1111/j.1439-0507.2006.01203.x
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Wickes,BL
中科院分区：
文献类型：
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作者：
Saracli,MA;Yildiran,ST;Sener,K;Gonlum,A;Doganci,L;Keller,SM;Wickes,BL
从1996年和1997年从土耳其54个不同省份采集的634份鸽子粪便样品中分离到26株环保型新生隐球菌。脉冲场凝胶电泳法(PFGE)结果表明，26株细菌可分为24种不同的脉冲场凝胶电泳型。在交配型研究中，26株菌株中有20株为MATα，4株为MATA，1株为MATA/α，1株为STE20不能分型。通过聚合酶链式反应分型，所有的分离物都是A型，这些分离物之间的广泛异质性表明，土耳其可能不存在单一的克隆群体。此外，α杂交株的存在可能表明土耳其环境中存在阿马塔交配型毒株。
A total of 26 environmentalCryptococcus neoformansvar.neoformansstrains isolated from 634 samples of pigeon droppings collected from 54 different provinces of Turkey in 1996 and 1997 were included in this study. The results of pulsed‐field gel electrophoresis (PFGE) showed that the 26 strains could be separated into 24 different PFGE patterns. In a mating‐type study, of 26 strains, 20 wereMATα, four wereMATa, one wasMATa/αand one was non‐typable by STE20 specific primers. By the polymerase chain reaction typing, all the isolates were serotype A. The extensive heterogeneity among these isolates suggests that a single clonal population may not be present in Turkey. Additionally, the presence of an AMATa/DMATαhybrid may indicate the existence of strains that are AMATa mating type in Turkish environment.