Molecular features of organic anion permeablity in ox red blood cell.
Molecular features of organic anion permeablity in ox red blood cell.
复制标题
牛红细胞有机阴离子通透性的分子特征。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
R. Motais
中科院分区：
文献类型：
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作者：
L. Aubert;R. Motais
1.在实验条件下，研究了有机阴离子渗透到牛红细胞中的情况，在实验条件下，它可以与通过膜的脂质区扩散进行的未解离酸的渗透区分开来。2.几条线的证据表明，进入的有机阴离子不能归因于简单的扩散通过水的通道限制的正电荷，但需要一个特定的相互作用的渗透阴离子与组件的膜。3.允许离子转移的结构要求是最小分子的强极性头部，以及链长大于C4的酸的两亲性结构。底物和受体之间的相互作用需要至少三点连接，涉及底物中的三个氧原子，其与受体上的互补位点反应以形成离子键和氢键。这种三点连接可以通过磺酸基或通过α酮取代、α羟基取代、添加酰胺基或添加空间上靠近第一羧基的第二羧基与羧酸进行。4.正如甲酸根阴离子的行为所表明的那样，在这样的转运系统中，任何羧酸都可以与受体瞬时相互作用，因此干扰有机阴离子的转运，即使这种与受体的离子相互作用不足以产生酸本身的转运。
1. The penetration of organic anions into bovine red blood cells has been studied under experimental conditions where it could be distinguished from the penetration of undissociated acids which proceeds by diffusion through lipid zones of the membrane. 2. Several lines of evidence suggest that the entry of organic anions cannot be ascribed to simple diffusion across aqueous channels limited by positive charges but needs a specific interaction of the penetrating anion with a component of the membrane. 3. The structural requirements allowing for ionic transfer is a strong polar head for the smallest molecules and in addition an amphiphilic structure for acids with chain length greater than C4. Interaction between substrate and receptor requires at least a three point attachment involving three oxygen atoms in the substrate which react with complementary loci on the receptor to form ionic and hydrogen bonds. Such a three point attachment can be made by a sulphonic group or with carboxylic acid by alpha ketosubstitution, alpha hydroxysubstitution, addition of an amidegroup or addition of a second carboxyl group spatially close to the first. 4. As suggested by the behaviour of the formate anion, in such a transport system any carboxylic acid could interact transiently with the receptor and therefore interfere with the transport of an organic anion even though such ionic interaction with the receptor were insufficient to produce transport of the acid itself.