LIVE VACCINE USED TO PREVENT SPREAD OF VARICELLA IN CHILDREN IN HOSPITAL
LIVE VACCINE USED TO PREVENT SPREAD OF VARICELLA IN CHILDREN IN HOSPITAL
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DOI：
10.1016/s0140-6736(74)90144-5
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发表时间：
1974-01-01
期刊：
影响因子：
168.9
通讯作者：
ISOMURA, S
中科院分区：
文献类型：
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作者：
TAKAHASH.M;OTSUKA, T;ISOMURA, S
将一株水痘病毒(Oka株)在人胚肺细胞中连续培养，然后在豚鼠胚胎(G.P.E.)中培养，使其减毒。细胞。皮下注射病毒刺激补体固定的产生(C.F.)正常易感儿童无临床反应的抗体。该病毒在人类二倍体(WI-38)细胞中繁殖，在G.P.E.细胞中传代后，在正常易感儿童中也能有效地诱导免疫反应，而没有临床反应。从G.P.E.细胞中提取的减毒病毒用于23名住院儿童，在儿童病房的一名患者出现水痘症状后，没有水痘病史，也没有检测到C.F.抗体。所有接种疫苗的儿童，包括接受类固醇治疗的儿童，都有抗体反应。除了一名未接种疫苗的患者出现一例严重病例外，没有发现任何麻烦的临床反应，水痘感染的传播也得到了防止。16名患有肾炎和肾病综合征的儿童也接种了疫苗。所有接种疫苗的儿童均观察到免疫应答，临床反应和实验室检查均未见异常。这些结果表明，制备水痘活疫苗是可能的，该疫苗既可以安全有效地用于高危儿童，也可以用于正常儿童。
A strain (Oka strain) of varicella virus was attenuated by cultivating it serially in human embryonic lung cells and then in guineapig embryo (G.P.E.) cells. Subcutaneous injection of the virus stimulated the production of complement-fixing (C.F.) antibody without clinical reactions in normal susceptible children. The virus propagated in human diploid (WI-38) cells after passage in G.P.E. cells was also effective in inducing an immunological response without clinical reactions in normal susceptible children. The attenuated virus derived from G.P.E. cells was used in 23 children in hospital with no history of varicella and no detectable C.F. antibody immediately after symptoms of varicella were found in a patient in the children's ward. There was an antibody response in all the vaccinated children including those who were receiving steroid therapy. No troublesome clinical reactions were noticed and the spread of varicella infection was prevented, with the exception of one severe case in an unvaccinated patient. 16 children with nephritis and nephrotic syndrome were also vaccinated. An immunological response was observed in all the vaccinated children, with no clinical reactions and no abnormal laboratory findings when blood and urine samples were examined. These results suggest that it is possible to prepare a live varicella vaccine which may be safely and effectively used for high-risk children as well as normal children.