Changes in gene expression levels and chloroplast anatomy induced by Leifsonia xyli subsp. xyli in sugarcane
Changes in gene expression levels and chloroplast anatomy induced by Leifsonia xyli subsp. xyli in sugarcane
复制标题
DOI：
10.1080/17429145.2021.2003881
复制
发表时间：
2021-01
影响因子：
3.2
通讯作者：
Xiaoqiu Zhang;Yongjian Liang;Xiu-Xia Song;Zeping Wang;Baoqing Zhang;Jingchao Lei;Yangrui Li;Meixin Yan
中科院分区：
文献类型：
--
作者：
Xiaoqiu Zhang;Yongjian Liang;Xiu-Xia Song;Zeping Wang;Baoqing Zhang;Jingchao Lei;Yangrui Li;Meixin Yan
【摘要】木莱氏菌引起的再生阻滞。木黎（xyli, Lxx）广泛分布于甘蔗种植区。本研究采用转录组学和细胞学方法分析接种Lxx后基因表达水平和叶绿体超微结构的变化。共获得90.26 Gb的干净碱基，156,713个单基因和406个差异表达基因。HSP和ACS基因持续差异表达。光合作用相关基因PsbO、PsbQ、PsaD、PsaE、PsaG和pah均上调。PIRH2、WRKY、NCED、AHP和GST是Lxx接种应答的重要特异性基因。接种Lxx后，甘蔗叶片叶绿体形态发生改变，膜遭到破坏。甘蔗对Lxx感染的反应可能是通过改变某些特定基因的表达水平和干扰光合作用。推测Lxx诱导的可能的分子调控网络。这些发现拓宽了对甘蔗与Lxx相互作用机制的认识。
ABSTRACT Ratoon stunt caused by Leifsonia xyli subsp. xyli (Lxx) is widely spread across sugarcane planting regions. Here, transcriptomics and cytology were conducted to analyze the changes in gene expression levels and chloroplast ultrastructures after Lxx inoculation. A total of 90.26 Gb clean bases, 156,713 unigenes and 406 differentially expressed genes were obtained. The genes of HSP and ACS were continuously differentially expressed. The photosynthesis-related genes of PsbO, PsbQ, PsaD, PsaE, PsaG and PsaH were up-regulated. PIRH2, WRKY, NCED, AHP and GST were identified as the important specific genes that responded to Lxx inoculation. Lxx inoculation also altered the shape and damaged the membrane of chloroplasts in sugarcane leaves. Sugarcane responds to Lxx infection likely via changing the expression levels of some specific genes and interfered with photosynthesis. A possible molecular regulation network induced by Lxx was speculated. These findings broaden the understanding to the mechanisms of the interaction between sugarcane and Lxx.