A microfluidic platform for 3-dimensional cell culture and cell-based assays
A microfluidic platform for 3-dimensional cell culture and cell-based assays
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DOI：
10.1007/s10544-006-9016-4
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发表时间：
2007-02-01
影响因子：
2.8
通讯作者：
Park, Je-Kyun
中科院分区：
文献类型：
--
作者：
Kim, Minseok S.;Yeon, Ju Hun;Park, Je-Kyun
本文报道了一种新的微流控平台，引入肽水凝胶，使生物相容性的微环境，以及实现原位细胞为基础的分析。通过白蛋白测定比较胶原复合材料、OPLA和Puramatrix支架对人肝癌细胞（HepG 2）的良好环境选择。选择的生物相容性自组装肽水凝胶，Puramatrix，流体动力学聚焦在微流控装置的主通道的中间，同时细胞被三维固定和封装，而无需任何额外的表面处理。将HepG 2细胞在聚二甲基硅氧烷（PDMS）微流体装置中三维培养4天。比较了微肽支架与普通培养皿培养的细胞形态及白蛋白分泌速率。通过将不同的试剂注射到肽支架的任一侧中，微流体装置还由于分子扩散而在肽支架上形成线性浓度梯度分布。基于这一特性，通过Triton X-100进行毒性试验。随着更高的毒物浓度梯度的形成，肽支架中细胞的死亡区越宽。这种微流控平台有助于在体内类似的三维微环境，并具有可靠的细胞为基础的筛选和分析，包括细胞毒性测试，实时细胞活力监测，和连续的剂量响应分析的应用潜力。
This paper reports a novel microfluidic platform introducing peptide hydrogel to make biocompatible microenvironment as well as realizing in situ cell-based assays. Collagen composite, OPLA and Puramatrix scaffolds are compared to select good environment for human hepatocellular carcinoma cells (HepG2) by albumin measurement. The selected biocompatible self-assembling peptide hydrogel, Puramatrix, is hydrodynamically focused in the middle of main channel of a microfluidic device, and at the same time the cells are 3-dimensionally immobilized and encapsulated without any additional surface treatment. HepG2 cells have been 3-dimensionally cultured in a poly(dimethylsiloxane) (PDMS) microfluidic device for 4 days. The cells cultured in micro peptide scaffold are compared with those cultured by conventional petri dish in morphology and the rate of albumin secretion. By injection of different reagents into either side of the peptide scaffold, the microfluidic device also forms a linear concentration gradient profile across the peptide scaffold due to molecular diffusion. Based on this characteristic, toxicity tests are performed by Triton X-100. As the higher toxicant concentration gradient forms, the wider dead zone of cells in the peptide scaffold represents. This microfluidic platform facilitates in vivo-like 3-dimensional microenvironment, and have a potential for the applications of reliable cell-based screening and assays including cytotoxicity test, real-time cell viability monitoring, and continuous dose-response assay.