The effect of the source pressure on the abundance of ions of noncovalent protein assemblies in an electrospray ionization orthogonal time-of-flight instrument
The effect of the source pressure on the abundance of ions of noncovalent protein assemblies in an electrospray ionization orthogonal time-of-flight instrument
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DOI：
10.1002/rcm.275
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发表时间：
2001-01-01
影响因子：
2
通讯作者：
Heck, AJR
中科院分区：
文献类型：
--
作者：
Tahallah, N;Pinkse, M;Heck, AJR
研究了电喷雾正交飞行时间质谱仪界面区气压升高对不同非共价蛋白质组装体离子强度的影响。升高界面区域中的压力通常导致高m/z离子的增强检测。发现最佳压力取决于离子的m/z值。这种压力效应应仔细解决有关的离子丰度在质谱的非共价蛋白质组装体的溶液相丰度。版权所有（C）2001约翰威利父子有限公司
The effect of elevating the pressure in the interface region of an electrospray ionization orthogonal time-of-flight mass spectrometer on the ion intensity of different noncovalent protein assemblies has been investigated. Elevating the pressure in the interface region generally led to an enhanced detection of high m/z ions. The optimum pressure was found to be dependent on the m/z value of the ions. This pressure effect should be carefully addressed when relating ion abundance in the mass spectra to solution phase abundance of noncovalent protein assemblies. Copyright (C) 2001 John Wiley & Sons, Ltd.