Serum cytokines are associated with n-3 polyunsaturated fatty acids and not with methylmercury measured in infant cord blood in the Seychelles child development study.
Serum cytokines are associated with n-3 polyunsaturated fatty acids and not with methylmercury measured in infant cord blood in the Seychelles child development study.
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DOI：
10.1016/j.envres.2021.112003
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发表时间：
2021-09
影响因子：
8.3
通讯作者：
T. Spence;A. Zavez;P. Allsopp;M. Conway;A. Yeates;M. Mulhern;E. van Wijngaarden;J. Strain;G. Myers;G. Watson;P. Davidson;C. Shamlaye;S. Thurston;E. McSorley
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作者：
T. Spence;A. Zavez;P. Allsopp;M. Conway;A. Yeates;M. Mulhern;E. van Wijngaarden;J. Strain;G. Myers;G. Watson;P. Davidson;C. Shamlaye;S. Thurston;E. McSorley
母亲食用鱼类会增加婴儿的甲基汞(MeHg)暴露和多不饱和脂肪酸(PUFA)浓度。N-3多不饱和脂肪酸是炎症的调节者，而甲基汞可能会影响脐带细胞因子的谱，从而促进免疫调节的结果。这项研究的目的是在调整脐带多不饱和脂肪酸的因素时，调查婴儿暴露于甲基汞与脐带细胞因子浓度之间的关系。方法我们研究了塞舌尔儿童发展研究(SCDS)营养队列2(NC2)的参与者，这是一个高吃鱼人群中的大型出生队列。于分娩时采集脐带血，测定全血甲基汞、血清多不饱和脂肪酸及血清细胞因子(干扰素-γ、IL-1β、IL-2、IL-12p70、肿瘤坏死因子-α、IL-4、IL-10、IL-13、IL-6、IL-8)浓度。线性回归检验了婴儿接触甲基汞与脐带细胞因子浓度之间的关系，并对脐带多不饱和脂肪酸进行了调整和不调整。通过交互作用模型检验了脐带血甲基汞、细胞因子和n-6：n-3比值(低/中/高)。结果无论是否调整多不饱和脂肪酸，脐带血甲基汞(34.08g/L)与细胞因子浓度之间均无总体关联。总n-3多不饱和脂肪酸(DHA、EPA和ALA)的升高与IL-10降低(β=−0.667；p=0.007)和总Th2(IL-4、IL-10和IL-13)的降低(β=−=0.715；p=0.036)显著相关。在交互作用模型中，甲基汞和IL-1β在n-6：n-3比值最低的三分位数中呈阳性，且与零有显著差异(β=0.002，p=0.03)。结论食用鱼类接触甲基汞不会影响脐带血中的炎症标志物。脐血n-3多不饱和脂肪酸与较低的IL-10和总Th2细胞因子的相关性提示，它们可能对炎症环境的调节有有益的影响。这些发现对公共卫生建议很重要，值得在后续研究中进行调查。
BackgroundMaternal fish consumption increases infant methylmercury (MeHg) exposure and polyunsaturated fatty acid (PUFA) concentrations. The n-3 PUFA are regulators of inflammation while MeHg may impact the cord cytokine profile and, subsequently, contribute to immune mediated outcomes. This study aimed to investigate associations between infant MeHg exposure and cord cytokine concentrations while adjusting for cord PUFA.MethodsWe studied participants in the Seychelles Child Development Study (SCDS) Nutrition Cohort 2 (NC2), a large birth cohort in a high fish-eating population. Whole blood MeHg, serum PUFA and serum cytokine concentrations (IFN-γ, IL-1β, IL-2, IL-12p70, TNF-α, IL-4, IL-10, IL-13, IL-6 and IL-8) were measured in cord blood collected at delivery (n = 878). Linear regression examined associations between infant MeHg exposure and cord cytokines concentrations, with and without adjustment for cord PUFA. An interaction model examined cord MeHg, cytokines and tertiles of the n-6:n-3 ratio (low/medium/high).ResultsThere was no overall association between cord MeHg (34.08 ± 19.98 μg/L) and cytokine concentrations, with or without adjustment for PUFA. Increased total n-3 PUFA (DHA, EPA and ALA) was significantly associated with lower IL-10 (β = −0.667; p = 0.007) and lower total Th2 (IL-4, IL-10 and IL-13) (β = −0.715; p = 0.036). In the interaction model, MeHg and IL-1β was positive and significantly different from zero in the lowest n-6:n-3 ratio tertile (β = 0.002, p = 0.03).ConclusionMethylmercury exposure from fish consumption does not appear to impact markers of inflammation in cord blood. The association of cord n-3 PUFA with lower IL-10 and total Th2 cytokines suggests that they may have a beneficial influence on the regulation of the inflammatory milieu. These findings are important for public health advice and deserve to be investigated in follow up studies.