Coinfection of the secondary symbionts, Hamiltonella defensa and Arsenophonus sp. contribute to the performance of the major aphid pest, Aphis gossypii (Hemiptera: Aphididae)
Coinfection of the secondary symbionts, Hamiltonella defensa and Arsenophonus sp. contribute to the performance of the major aphid pest, Aphis gossypii (Hemiptera: Aphididae)
复制标题
DOI：
10.1111/1744-7917.12603
复制
发表时间：
2020-02-01
期刊：
影响因子：
4
通讯作者：
Mehrabadi, Mohammad
中科院分区：
文献类型：
--
作者：
Ayoubi, Aida;Talebi, Ali Asghar;Mehrabadi, Mohammad
细菌内共生体对蚜虫的生态学特性具有重要作用。在这项研究中，我们的特点是细菌内共生体的A。在伊朗卡拉杰采集的棉蚜及其在蚜虫表现中的作用。结果表明，除蚜生布氏霉（Buchnera aphidicola）外，蚜生布氏霉（A.定量PCR（qPCR）结果显示，在若虫发育至成虫羽化的整个过程中，内共生菌的数量不断增加;此后，Buchnera和Arsenophonus的数量逐渐减少，而H.防守不断加强。Buchnera减少导致延长的发展和没有后代生产。此外，次级共生体减少导致总寿命和自然增长的内在速率以及畸形死亡的后代的外观相比，对照昆虫的减少。减少次生共生体并不影响苜蓿蚜茧蜂对苜蓿蚜的寄生率。这些结果表明，H。defensa和Arsenophonus对A.棉蚜对寄生蜂的抗性不是通过提高其生产性能而实现的。
Bacterial endosymbionts play important roles in ecological traits of aphids. In this study, we characterize the bacterial endosymbionts of A. gossypii collected in Karaj, Iran and their role in the performance of the aphid. Our results indicated that beside Buchnera aphidicola, A. gossypii, also harbors both Hamiltonella defensa and Arsenophonus sp. Quantitative PCR (qPCR) results revealed that the populations of the endosymbionts increased throughout nymphal development up to adult emergence; thereafter, populations of Buchnera and Arsenophonus were diminished while the density of H. defensa constantly increased. Buchnera reduction caused prolonged development and no progeny production. Furthermore, secondary symbiont reduction led to reduction of the total life span and intrinsic rate of natural increase as well as appearance of the deformed dead offspring in comparison with the control insects. Reduction of the secondary symbionts did not affect parasitism rate of the aphid by the parasitic wasp Aphidius matricariae. Together these findings showed that H. defensa and Arsenophonus contributed to the fitness of A. gossypii by enhancing its performance, but not through parasitoid resistance.