Improving frequency of thrombosis by altering blood flow in the carrageenan-induced rat tail thrombosis model
Improving frequency of thrombosis by altering blood flow in the carrageenan-induced rat tail thrombosis model
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DOI：
10.1016/j.phrs.2009.04.010
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发表时间：
2009-10-01
影响因子：
9.3
通讯作者：
Arakawa, Masayuki
中科院分区：
文献类型：
--
作者：
Hagimori, Masayori;Kamiya, Seitaro;Arakawa, Masayuki
在大鼠和小鼠中的角叉菜胶诱导的尾部血栓形成模型作为体内模型可用于在药物发现项目中评价具有抗血栓形成作用的化合物。然而，血栓形成的频率较低，并且在标准条件下血栓形成的迹象并不恒定。在这项研究中，我们修改了这个模型的基础上高凝状态，内皮损伤，并改变正常的血流。简单结扎以改变大鼠的血流显著改善静脉注射κ-角叉菜胶后血栓形成的频率和体征。特别是，1 mg/kg κ-角叉菜胶注射与10分钟尾部结扎相结合，使大鼠中血栓形成的频率增加至几乎100%。这些结果表明，这种改进的模型具有很大的优势，比以前使用的条件下，用于评估抗血栓和溶栓药物的影响。(C)2009爱思唯尔有限公司保留所有权利。
The carrageenan-induced tail thrombosis model in rats and mouse is useful for evaluating compounds having antithrombotic effects in drug discovery projects as an in vivo model. However, the frequency of thrombosis is low, and the signs of thrombosis are not constant under the standard conditions. In this study, we modified this model on the basis of hypercoagulability, endothelial injury, and alteration in the normal blood flow. The simple ligation to alter blood flow in rats significantly improved the frequency and signs of thrombosis after intravenous kappa-carrageenan injection. In particular, 1 mg/kg kappa-carrageenan injection in combination with 10 min ligation at the tail increased the frequency of thrombosis to almost 100% in rats. These results suggest that this modified model has great advantages over the previously used conditions for evaluating the effects of antithrombotic and thrombolytic agents. (C) 2009 Elsevier Ltd. All rights reserved.