Correlation of bacterial hyperthermic survival with anaesthetic potency.
Correlation of bacterial hyperthermic survival with anaesthetic potency.
复制标题
细菌高温存活与麻醉效力的相关性。
DOI：
10.1080/09553008214551011
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Dennis,WH
中科院分区：
文献类型：
--
作者：
Yatvin,MB;Gipp,JJ;Rusy,BF;Dennis,WH
我们已经评估了几种局部麻醉药和催眠药影响高温细胞杀伤的相对能力。将暴露于热和麻醉剂后的细菌细胞存活用作测定系统。使用的大肠杆菌是不饱和脂肪酸营养缺陷型K1060。它在37°C下在补充有油酸的培养基中生长，然后在存在麻醉剂的情况下暴露于47°C的高温。局部麻醉药试验普鲁卡因，利多卡因，丁卡因，和苯并咪唑，和全身麻醉药巴比妥和戊巴比妥。在5小时的加热时间内测定每种麻醉剂的剂量反应，在加热期间使单独加热时发现的细胞杀伤时间减半所需的麻醉剂浓度为普鲁卡因5.9 mM，利多卡因0.8 mM，丁卡因0.12 mM，苯并咪唑2.0 mM，巴比妥6.7 mM和戊巴比妥1.2 mM。局部麻醉剂的等效效应剂量与相同麻醉剂的相对效力（通过小鼠呼吸停止和犬心肌收缩力测定）的已发表数据之间存在直接相关性，我们所描述的测定将是这些药物与高热相互作用的方便和简单的测试。在联合放疗-热疗治疗中，这种与热疗的相互作用作为一种辅助手段的使用应加以检验。
We have evaluated several local anaesthetics and hypnotics for their relative ability to influence hyperthermic cell killing. Bacterial cell survival following exposure to heat and anaesthetic was used as the assay system.TheE. colibacterium used was the unsaturated fatty acid auxotroph, K1060. It was grown at 37°C in medium supplemented with oleic acid and then exposed to 47°C hyperthermia in the presence of an anaesthetic. The local anaesthetics tested were procaine, lidocaine, tetracaine, and benzocaine, and the general anaesthetics were barbital and pentobarbital. The dose response for each anaesthetic was determined over a five-hour heating period.The anaesthetic concentration required during heating to halve the time for cell killing found with heat alone is 5·9 mM for procaine, 0·8 mM for lidocaine, 0·12 mM for tetracaine, 2·0 mM for benzocaine, 6·7 mM for barbital and 1·2 mM for pentobarbital. There is a direct correlation between equivalent effect doses of the local anaesthetics and published data for the relative potency of the same anaesthetics as determined by respiratory arrest in mice and by myocardial contractile force in dogs.The assay we have described would be a convenient and easy test for the interaction of these drugs with hyperthermia. The use of this interaction with hyperthermia as an adjuvant in combined radiation-hyperthermia therapy should be tested.
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影响因子：
8.8
作者：
F. Foldes;D. Davis;O. J. Plekss
通讯作者：
O. J. Plekss
大肠杆菌渗透压休克细胞中半乳糖转运的减少和恢复。
DOI：
10.1016/s0021-9258(18)96195-8
发表时间：
1967
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Y. Anraku
通讯作者：
Y. Anraku
膜脂成分和普鲁卡因对细胞高温死亡的影响。
DOI：
--
发表时间：
1977
期刊：
International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine
影响因子：
--
作者：
M. Yatvin
通讯作者：
M. Yatvin
影响因子：
8.8
作者：
J. Steinhaus
通讯作者：
J. Steinhaus
影响因子：
8.8
作者：
D. M. Stewart;W. Rogers;J. E. Mahaffey;S. Witherspoon;E. F. Woods
通讯作者：
E. F. Woods