Sumoylation in axons triggers retrograde transport of the RNA-binding protein La
Sumoylation in axons triggers retrograde transport of the RNA-binding protein La
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DOI：
10.1073/pnas.0611562104
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发表时间：
2007-07-31
影响因子：
11.1
通讯作者：
Twiss, Jeffery L.
中科院分区：
文献类型：
--
作者：
van Niekerk, Erna A.;Willis, Dianna E.;Twiss, Jeffery L.
一个令人惊讶的大群体的mRNA已被证明本地化的感觉轴突，但很少RNA结合蛋白已在这些轴突中检测到。这些轴突mRNA包括La RNA伴侣蛋白的几个潜在的结合靶标。镧被运输到轴突过程中的文化和周围神经。有趣的是，La在感觉神经元中通过sumoylation进行后修饰。在轴突中，小泛素样修饰多肽（SUMO）-La与动力蛋白相互作用，而天然La与驱动蛋白相互作用。赖氨酸41是sumoylation所需的，sumoylation无能的La-K41 R只显示顺行运输，而WT La显示轴突中的顺行和逆行运输。因此，SUMOylation的La决定了其运输的方向性轴突室内，SUMO-La可能回收到细胞体。
A surprisingly large population of mRNAs has been shown to localize to sensory axons, but few RNA-binding proteins have been detected in these axons. These axonal mRNAs include several potential binding targets for the La RNA chaperone protein. La is transported into axonal processes in both culture and peripheral nerve. Interestingly, La is posttranslationally modified in sensory neurons by sumoylation. In axons, small ubiquitin-like modifying polypeptides (SUMO)-La interacts with dynein, whereas native La interacts with kinesin. Lysine 41 is required for sumoylation, and sumoylation-incompetent La-K41R shows only anterograde transport, whereas WT La shows both anterograde and retrograde transport in axons. Thus, sumoylation of La determines the directionality of its transport within the axonal compartment, with SUMO-La likely recycling to the cell body.