The Rasgrf1-repeat sequence (D9Ncvs53) maps between Mod1 and Rbp1 on mouse chromosome 9 and may define a putative imprinted region.
The Rasgrf1-repeat sequence (D9Ncvs53) maps between Mod1 and Rbp1 on mouse chromosome 9 and may define a putative imprinted region.
复制标题
Rasgrf1 重复序列 (D9Ncvs53) 位于小鼠 9 号染色体上的 Mod1 和 Rbp1 之间，并且可以定义推定的印记区域。
DOI：
10.1007/s003359900741
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Plass,C
中科院分区：
文献类型：
--
作者：
Pearsall,RS;Imai,K;Shibata,H;Hayashizaki,Y;Chapman,VM;Held,WA;Plass,C
作图方法：由94只动物组成的公开可用的BSS-杰克逊回交组的单倍型分析。有关资料可于网页（www. Jax. org/resources/documents/cmdata/BSS. html）。数据库存款信息：Sp 4克隆可以在Genbank数据库中以登录号136746找到。分子试剂：使用含有直接串联重复序列[1]的RLGS斑点克隆Sp 4作为Southern印迹分析的探针。Sp 4是从Notl连接文库分离的2.0-kb NotlMBol克隆（Genbank登录号136746）。用于SSLP分析的引物获自Research Genetics（Huntsville，Ala.）。D9 Mit 33位于Rbpl的5 '非翻译区（UTR），D9 Mit 24扩增转铁蛋白（Trf）基因。Mod]用引物对#2和#3作图，引物对#2和#3从Mod]基因的3 'UTR扩增373-bp片段（位置2529-2901）[2]。Mod]# 2：5'-GTA CCC OTT TTA OCT CAC ACC-3 '; Mod]# 3：阿加CAA OCT CAG GCC TCC ATG-3'。等位基因检测：以Sp 4为杂交探针的Southern印迹分析鉴定了来自C57 BL 16的2.2-kb EcoRI片段和来自Mus spretus的2.3-kb EcoRI片段。此外，通过EcoRV消化来自各种小鼠品系的DNA鉴定了六个等位基因（M. caroli= 1.5kb; PWK= 1.6kb; M. hortulanus，M. macedonicus= 1.7kb; C57BL/6，M. castaneus= 2.1kb; DBA= 2.3kb; M. spretus= 18 kb）。在先前描述的条件下用引物#2/#3进行Modl的PCR分型[2]。用TaqI消化PCR产物以产生来自C57 BL/6的373-bp片段和来自C57 BL/6的250+ 120 bp片段。
Method of mapping: Haplotype analysis of the publicly available BSS-Jackson backcross panel consisting of 94 animals. The typings are available at the website (www. jax. org/resources/documents/cmdata/BSS. html). Database deposit information: The Sp4 clone can be found in the Genbank database under the Accession Number 136746. Molecular reagents: An RLGS spot clone, Sp4, containing a direct tandem repeat [1] was used as a probe in Southern blot analysis. Sp4 is a 2.0-kb NotlIMbol clone isolated from a Notl linking library (Genbank Accession Number 136746). Primers for SSLP analysis were obtained from Research Genetics (Huntsville, Ala.). D9Mit33 is found in the 5'untranslated region (UTR) of Rbpl, and D9Mit24 amplifies the transferrin (Trf) gene. Mod] was mapped with the primer pair# 2 and# 3 which amplifies a 373-bp fragment (position 2529-2901) from the 3'UTR of the Mod] gene [2]. Mod]# 2: 5'-GTA CCC OTT TTA OCT CAC ACC-3'; Mod]# 3: 5'-AGA CAA OCT CAG GCC TCC ATG-3'. Allele detection: Southern blot analysis with Sp4 as a hybridization probe identified a 2.2-kb EcoRI fragment from C57BL16 and a 2.3-kb EcoRI fragment from Mus spretus. In addition, six alleles were identified by EcoRV digestion of DNA from various mouse strains (M. caroli= 1.5 kb; PWK= 1.6 kb; M. hortulanus, M. macedonicus= 1.7 kb; C57BL/6, M. castaneus= 2.1 kb; DBA= 2.3 kb; and M. spretus= 18 kb). PCR typing of Modl with primers# 2/# 3 was performed under previously described conditions [2]. The PCR products were digested with TaqI to yield a 373-bp fragment from C57BL/6 and 250+ 120 bp fragments from