Slow-freezing cryopreservation of neural stem cell spheres with different diameters
Slow-freezing cryopreservation of neural stem cell spheres with different diameters
复制标题
不同直径神经干细胞球的慢冻冷冻保存
DOI：
10.1016/j.cryobiol.2009.10.013
复制
发表时间：
2010-04-01
期刊：
影响因子：
2.7
通讯作者：
Cui, Zhan-Feng
中科院分区：
文献类型：
--
作者：
Ma, Xue-Hu;Shi, Ying;Cui, Zhan-Feng
神经干细胞(NSCs)具有重要的临床应用和科研价值，发展高效的低温保存方案可以极大地方便临床应用的储存和运输。本研究的目的是提高单细胞悬液、直径为30~50µm和80~100µm的神经干细胞微球分别用二甲基亚砜(Me2SO)低温保护剂(CPA)缓慢冷冻保存的神经干细胞的存活率和活力。然后对解冻后的神经干细胞进行存活率和分化能力检测。直径为80-100微米、Me2SO浓度为8%的NSC球的存活率为82.9%。这些结果表明，神经干细胞之间的微妙相互作用和球径可能共同影响存活率(C)2009 Elsevier Inc.版权所有
Neural stem cells (NSCs) are of great value for clinical application and scientific research The development of efficient cryopreservation protocols could significantly facilitate the storage and transportation for clinic applications The objective of the present study is to improve the survival rate and viability of NSCs Neural stein cells with three states of single-cell suspension, NSC spheres with diameters of 30-50 mu m and 80-100 mu m, were cryopreserved by slow-freezing method with the cryoprotective agent (CPA) of dimethyl sulfoxide (Me2SO), respectively. Then the post-thawing NSCs were tested for the survival rate and the differentiation ability As a result. NSC spheres with diameter of 80-100 mu m and Me2SO concentration of 8% achieve the survival rate of 82.9%. and the NSCs still sustain the multi-differentiation potentiality These results indicated that both the subtle interaction among NSCs and sphere diameters may affect the survival rate together (C) 2009 Elsevier Inc All rights reserved