MAP OF SEQUENTIAL B-CELL EPITOPES OF THE HIV-1 TRANSMEMBRANE PROTEIN USING HUMAN-ANTIBODIES AS PROBE
MAP OF SEQUENTIAL B-CELL EPITOPES OF THE HIV-1 TRANSMEMBRANE PROTEIN USING HUMAN-ANTIBODIES AS PROBE
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DOI：
10.1159/000150169
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发表时间：
1990-11-01
期刊：
影响因子：
4.6
通讯作者：
BRASSEUR, R
中科院分区：
文献类型：
--
作者：
GOUDSMIT, J;MELOEN, RH;BRASSEUR, R
使用感染人类免疫缺陷病毒1型（HIV-1）的个体的抗体，通过抗体反应性肽扫描（Pepscan）来探测41 kD的HIV-1跨膜蛋白（gp 41）的抗原性。11个不同的连续抗体结合位点的定义，通过测试339重叠的九肽跨越完整的gp 41氨基酸序列的反应性。这种分析仅映射长度为9个氨基酸的连续抗体结合位点，并且不鉴定推定的不连续或组装的表位。3个B细胞表位（aa 609-622; aa 655-699; aa 664-681）位于推定的跨膜锚的氨基端边界，2个（aa 732-748; aa 744-762）位于该结构域的羧基端边界，是最具抗原性的。一个抗体结合域（aa 834-852）的4个氨基酸同源的HLA II类β-链的β-1域被识别的血清中的1/4的艾滋病患者测试，而不是任何的8个血清中的无HIV-1的个人。尽管参与病毒复制、细胞病变和可能的免疫抑制的gp 41的功能域被证明与HIV-1感染者的抗体结合，但抗体识别模式与疾病进展之间没有明显的关系。
Antibodies of individuals infected with the human immunodeficiency virus type 1 (HIV-1) were used to probe the antigenicity of the HIV-1 transmembrane protein of 41 kD (gp41) by antibody-reactive peptide scanning (Pepscan). Eleven distinct sequential antibody-binding sites were defined by testing reactivity to 339 overlapping nonapeptides spanning the complete gp41 amino acid sequence. Such analysis only maps continuous antibody-binding sites of nine amino acids in length and does not identify putative discontinuous or assembled epitopes. Three B cell epitopes (aa 609-622; aa 655-699; aa 664-681) at the amino-terminal border of the putative transmembrane anchor and two (aa 732-748; aa 744-762) at the carboxyl-terminal border of this domain were the most antigenic. One antibody-binding domain (aa 834-852) with four amino acids homologous to the beta-1 domain of HLA class II beta-chain was recognized by the serum in 1 of 4 AIDS patients tested and not by any of the eight sera from symptom-free individuals. Although functional domains of gp41 involved in virus replication, cytopathicity and possibly immunossuppression were shown to bind antibodies of HIV-1-infected individuals, no relationship between antibody recognition patterns and disease progression was apparent.