Two distinct cellular phosphoproteins bind to the c-fos serum response element.
Two distinct cellular phosphoproteins bind to the c-fos serum response element.
复制标题
两种不同的细胞磷蛋白与 c-fos 血清反应元件结合。
DOI：
10.1002/j.1460-2075.1989.tb03572.x
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Gilman,MZ
中科院分区：
文献类型：
--
作者：
RyanJr,WA;FranzaJr,BR;Gilman,MZ
血清生长因子诱导c-fos转录需要一个20 bp的DNA序列，称为血清反应元件（SRE）。SRE是一种核蛋白，血清反应因子（SRF）的结合位点。目前还没有直接证据表明SRF活动与SRE功能之间的联系。我们在这里显示，细胞含有第二种特异性SRE结合蛋白。它与SRF的区别在于其大小、色谱特性、结合特异性和与SRE中核苷酸的接触。细胞中存在两种不同的蛋白质，可以独立地结合到SRE表明，SRE的多种功能可能是由不同的蛋白质介导的。
Induction of c‐fos transcription by serum growth factors requires a 20 bp DNA sequence termed the serum response element (SRE). The SRE is a binding site for a nuclear protein, serum response factor (SRF). There is as yet no direct evidence that links SRF activity to SRE function. We show here that cells contain a second specific SRE‐binding protein. It is distinguished from SRF by its size, chromatographic properties, binding specificity and contacts with nucleotides in the SRE. The presence in cells of two distinct proteins that can bind independently to the SRE suggests that the multiple functions of the SRE may be mediated by distinct proteins.