LOCATION OF THE MAJOR ANTIGENIC SITES INVOLVED IN ROTAVIRUS SEROTYPE-SPECIFIC NEUTRALIZATION
LOCATION OF THE MAJOR ANTIGENIC SITES INVOLVED IN ROTAVIRUS SEROTYPE-SPECIFIC NEUTRALIZATION
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DOI：
10.1073/pnas.83.10.3465
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
HOLMES, IH
中科院分区：
文献类型：
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作者：
DYALLSMITH, ML;LAZDINS, I;HOLMES, IH
轮状病毒的主要外壳糖蛋白的抗原位点，确定了使用突变体病毒选择的耐中和（型特异性）单克隆抗体。对来自这些突变体的糖蛋白基因进行测序以确定蛋白质中所得氨基酸取代的位置和性质。鉴定了三个区域（A、B和C）（分别为氨基酸87-96、145-150和211-223），其中C区似乎是最重要的。C区的突变导致对多克隆抗病毒抗血清中和的抗性增加10倍。本研究的结果以及其他数据表明，天然蛋白质的三维折叠使得区域A和C非常接近。
Antigenic sites on the rotavirus major outershell glycoprotein were identified by using mutant viruses selected for resistance to neutralizing (serotype-specific) monoclonal antibodies. The glycoprotein genes from these mutants were sequenced to determine the position and nature of the resultant amino acid substitutions in the protein. Three regions (A, B, and C) were identified (amino acids 87-96, 145-150, and 211-223, respectively), of which region C appears to be the most important. A mutation in region C caused a 10-fold increase in resistance to neutralization by polyclonal antiviral antiserum. The results of this study, together with other data, indicate that the three-dimensional folding of the native protein is such that regions A and C are in close proximity.