Effect of short-term temperature stress on fruit set and the expression of an auxin reporter gene and auxin synthesis genes in tomato
Effect of short-term temperature stress on fruit set and the expression of an auxin reporter gene and auxin synthesis genes in tomato
复制标题
DOI：
10.1016/j.scienta.2022.111039
复制
发表时间：
2022-03-21
影响因子：
4.3
通讯作者：
Kato, Kazuhisa
中科院分区：
文献类型：
--
作者：
Ito, Hikaru;Kanayama, Yoshinori;Kato, Kazuhisa
在番茄栽培中，温度胁迫是造成坐果和生长不良的主要原因。我们重点研究了短期温度胁迫对花前5天内每一天的影响。以表达生长素报告基因DR5::GUS的番茄品种Micro-Tom为模型系统，研究了温度胁迫与生长素相关因子的关系。DR5::GUS在对照植株的子房和花梗中表达量在发育后期有所增加，但在高温胁迫下没有表达或表达量极低。由于DR5::GUS基因表达水平较低，在高温胁迫下也能长出较小的果实。因此，本研究中任何阶段的暂时性高温胁迫都会影响生长素在子房和花序梗的定位，进而影响坐果和生长。结果表明，低温胁迫下，果实的坐果量没有减少，果实生长有一定的延迟和恢复，这与DR5::GUS的表达水平相对应。与生长素运输有关的番茄PIN基因启动子GUS的表达不受高温胁迫的影响。另一方面，与生长素合成有关的ToFZY基因的表达受到温度胁迫的影响，这可能是高温胁迫下DR5::GUS表达降低的部分机制。
Poor fruit set and growth are known to be caused by temperature stress in tomato cultivation. We focused on the effect of short-term temperature stress on each day during the five days before anthesis. The relationship between temperature stress and auxin-related factors was investigated using an experimental tomato cultivar, Micro-Tom expressing the auxin reporter DR5::GUS as a model system. While DR5::GUS expression in the control plants increased in ovaries and transferred to peduncles during the later developmental stages, that under high-temperature stress was not detected or very low. Some smaller fruit grew even under high-temperature stress probably due to the low level of DR5::GUS expression. Therefore, it was suggested that temporary high-temperature stress at any stage in this study affected the localization of auxin to ovaries and peduncles, and further affected fruit set and growth. By contrast, no decreased fruit set was observed with some delay and recovery of fruit growth under low-temperature stress, and this corresponded to the levels of DR5::GUS expression. The expression of GUS with the promoter of the tomato PIN gene, which is involved in the auxin transport, was not affected by high-temperature stress. On the other hand, the expression of ToFZY genes involved in auxin synthesis were affected by temperature stress, suggesting a part of the mechanism of the decrease in DR5::GUS expression under high-temperature stress.