Comparative analysis of biochemical properties of mesophyll and bundle sheath chloroplasts from various subtypes of C4 plants grown at moderate irradiance.
Comparative analysis of biochemical properties of mesophyll and bundle sheath chloroplasts from various subtypes of C4 plants grown at moderate irradiance.
复制标题
中等辐照度下生长的 C4 植物不同亚型叶肉和束鞘叶绿体生化特性的比较分析。
DOI：
10.18388/abp.2006_3298
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发表时间：
2006
影响因子：
1.7
通讯作者：
A. Drożak
中科院分区：
文献类型：
--
作者：
E. Romanowska;A. Drożak
以玉米（NADP-ME型）、黍（NAD-ME型）和大黍（PEP-CK型）为材料，研究了C4植物叶肉和维管束鞘叶绿体的光化学特性。本工作的目的是获得信息的选择光化学特性的叶肉和维管束鞘叶绿体分离C4植物生长在相同的温和的光条件。采用酶法和机械法分离维管束鞘叶绿体。在Z.玉米和大孢霉的酶法分离导致了部分类囊体多肽的损失。结果表明，不同植物叶肉和维管束鞘叶绿体的PSI和PSII活性存在显著差异，这种差异与叶绿体色素蛋白复合物的组成、光合磷酸化效率和荧光发射特性有关。这是首次报道C4亚型叶肉叶绿体光化学活性的差异。我们的研究结果还表明，叶肉和维管束鞘叶绿体在相同的光照条件下生长的C4植物的主要类囊体复合物的活性显着不同，这表明在C4亚型的光化学活性的发展中的作用的因素（S）以外。
The photochemical characteristics of mesophyll and bundle sheath chloroplasts isolated from the leaves of C4 species were investigated in Zea mays (NADP-ME type), Panicum miliaceum (NAD-ME type) and Panicum maximum (PEP-CK type) plants. The aim of this work was to gain information about selected photochemical properties of mesophyll and bundle sheath chloroplasts isolated from C4 plants grown in the same moderate light conditions. Enzymatic as well as mechanical methods were applied for the isolation of bundle sheath chloroplasts. In the case of Z. mays and P. maximum the enzymatic isolation resulted in the loss of some thylakoid polypeptides. It was found that the PSI and PSII activities of mesophyll and bundle sheath chloroplasts of all species studied differed significantly and the differences correlated with the composition of pigment-protein complexes, photophosphorylation efficiency and fluorescence emission characteristic of these chloroplasts. This is the first report showing differences in the photochemical activities between mesophyll chloroplasts of C4 subtypes. Our results also demonstrate that mesophyll and bundle sheath chloroplasts of C4 plants grown in identical light conditions differ significantly with respect to the activity of main thylakoid complexes, suggesting a role of factor(s) other than light in the development of photochemical activity in C4 subtypes.