EFFECTS OF LONG-CHAIN ACYL CARNITINE ON MEMBRANE FLUIDITY OF HUMAN-ERYTHROCYTES
EFFECTS OF LONG-CHAIN ACYL CARNITINE ON MEMBRANE FLUIDITY OF HUMAN-ERYTHROCYTES
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DOI：
10.1016/0005-2736(89)90318-0
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发表时间：
1989-04-28
期刊：
影响因子：
--
通讯作者：
YAMAZAKI, N
中科院分区：
文献类型：
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作者：
WATANABE, H;KOBAYASHI, A;YAMAZAKI, N
两亲性化合物，如长链酰基肉碱，在缺血心肌中积聚，并可能导致心肌损伤。为了表征棕榈酰肉碱引起的膜分子动力学的变化，用5-羟基硬脂酸对人红细胞膜进行了自旋标记，并通过测量ESR谱中有序参数的变化来定量膜流动性。棕榈酰卡尼汀引起人红细胞膜流动性的三相改变。膜流动性先升高5min，然后呈浓度依赖性降低。在较高浓度的棕榈酰肉碱(100nad 150微米)作用下，膜流动性分别在孵育30分钟和20分钟后再次增加。加入2.8 mM CaCl2可显著降低膜脂流动性，并增强棕榈酰卡尼汀对膜脂流动性的影响。这些结果提示，分子动力学的改变是长链丙烯肉碱在脑缺血损伤中发挥重要作用的机制之一。
Amphiphilic compounds such as long-chain acyl carnitines accumulate in ischemic myocardium and potentially contribute to the myocardial damage. To characterize alterations in membrane molecular dynamics produced by palmitoylcarnitine, human erythrocytes were spin-labeled with 5-doxylstearic acid, and membrane fluidity was quantified by measuring the changes in the order parameter derived from ESR spectra. Palmitoylcarnitine induced triphasic alterations in membrane fluidity of human erythrocytes. The membrane fluidity increased for 5 min, then decreased in a concentration-dependent manner. At higher concentrations (100 nad 150 .mu.M) of palmitoylcarnitine, membrane fluidity increased again after 30 and 20 min of the incubation, respectively. Addition of 2.8 mM CaCl2 resulted in a significant decrease in membrane fluidity and enhanced the alterations in membrane fluidity caused by palmitoylcarnitine. The results suggest that alterations in molecular dynamics are one mechanism through which long-chain acryl carnitine could play an important role in ischemic injury.