The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system
The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system
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DOI：
10.1111/imb.12119
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发表时间：
2014-12
影响因子：
2.6
通讯作者：
P. Pregitzer;M. Greschista;H. Breer;J. Krieger
中科院分区：
文献类型：
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作者：
P. Pregitzer;M. Greschista;H. Breer;J. Krieger
雄性飞蛾对雌性释放的性信息素具有非凡的敏感度。这种非凡的感觉能力是基于毛发样感觉细胞淋巴中的信息素结合蛋白和感觉神经元树突中的信息素受体的协同相互作用。在这里，我们研究了所谓的“感觉神经元膜蛋白1”（SNMP1）是否有助于对信息素成分(Z)‐11‐十六烯醛（Z11‐16:Ald）的反应。通过免疫组织化学和原位杂交，我们证明SNMP1实际上存在于表达Z11‐16:Ald受体HR13和感觉神经元树突的细胞中。为了评估SNMP1可能的功能，我们通过钙成像监测了单独表达HR13或组合表达SNMP1/HR13的细胞系对Z11‐16:Ald刺激的反应性。结果发现，SNMP1/HR13细胞对信息素刺激的敏感性是HR13细胞的1000倍。相比之下，表达HR13和非神经元型SNMP2的细胞在信息素敏感性方面没有变化。总的来说，我们的重构实验表明，SNMP1的存在显著增加了细胞对Z11‐16:Ald的基于HR13的反应性，这表明SNMP1也有助于天线神经元的反应，从而提高了信息素检测系统的显著敏感性。
Male moths detect female‐released sex pheromones with extraordinary sensitivity. The remarkable sensory ability is based on a cooperative interplay of pheromone binding proteins in the lymph of hair‐like sensilla trichodea and pheromone receptors in the dendrites of sensory neurones. Here we examined whether in Heliothis virescens the so‐called ‘sensory neurone membrane protein 1’ (SNMP1) may contribute to responsiveness to the pheromone component, (Z)‐11‐hexadecenal (Z11‐16:Ald). By means of immunohistochemistry and in situ hybridization we demonstrated that SNMP1 is in fact present in cells expressing the Z11‐16:Ald receptor HR13 and the dendrites of sensory neurones. To assess a possible function of SNMP1 we monitored the responsiveness of cell lines that expressed HR13 alone or the combination SNMP1/HR13 to stimulation with Z11‐16:Ald by calcium imaging. It was found that SNMP1/HR13 cells were 1000‐fold more sensitive to pheromone stimulation compared with HR13 cells. In contrast, cells that expressed HR13 and the non‐neuronal SNMP2‐type showed no change in pheromone sensitivity. Overall, our reconstitution experiments demonstrate that the presence of SNMP1 significantly increases the HR13‐based responsiveness of cells to Z11‐16:Ald, suggesting that SNMP1 also contributes to the response of the antennal neurones and thus to the remarkable sensitivity of the pheromone detection system.