Endurance training enhances ABCA1 expression in rat small intestine
Endurance training enhances ABCA1 expression in rat small intestine
复制标题
DOI：
10.1007/s00421-009-1133-3
复制
发表时间：
2009-10-01
影响因子：
3
通讯作者：
Abednazari, Hossein
中科院分区：
文献类型：
--
作者：
Khabazian, Behzad Mehdi;Ghanbari-Niaki, Abbass;Abednazari, Hossein
本研究旨在探讨大鼠在跑步训练过程中肝脏和肠道ABCA 1的表达及血浆HDL-C水平的变化。成年Wistar雄性大鼠20只（17-18周龄，体重300322 g），随机分为对照组（n = 10）和训练组（n = 10）。训练组以25 m/min（0%等级）的速度训练，60 min/d，5 d/周，共12周。在最后一次训练后48 h处死大鼠。与对照组相比，训练组小肠和肝脏ABCA 1 mRNA表达显著增加（P < 0.006和P < 0.024）。肠和肝脏ATP浓度保持不变。运动组血浆HDL-C、HDL 2-C、Apo A-1、前β HDL-C浓度、LCAT活性、TC/HDL-C、LDL-C/HDL-C比值均显著升高（P < 0.01、P < 0.006、P <0.001、P <0.001、P < 0.067、P < 0.02、P < 0.03）。然而，其他脂蛋白浓度不变。结果表明，耐力训练可显著升高大鼠血浆HDL-C和HDL 2-C浓度，并伴随着血浆Apo A-1、前β HDL-C浓度、LCAT活性和ABCA 1 mRNA表达水平的升高。
The purpose of this study was to investigate liver and intestinal ABCA1 expression and plasma HDL-C level in response to treadmill-running training in rats. Twenty adult Wistar male rats (17-18 weeks old, 300322 g) were divided into control (n = 10) and Training ( n = 10) groups. Training group trained at 25 m/min (0% grade) for 60 min/day, 5 days/week for 12 weeks. Rats were killed 48 h after the last session of training. The intestinal and liver ABCA1 mRNA expression was found to be significantly higher in trained compared to control group (P < 0.006 and P < 0.024, respectively). Intestine and liver ATP concentrations remained unchanged. Plasma HDL-C, HDL2-C, Apo A-1, pre-beta HDL-C concentration, LCAT activity, TC/HDL-C and LDL-C/HDL-C ratio significantly increased in trained group (P < 0.01, P < 0.006, P < 0.001, P < 0.001 P < 0.067, P < 0.02, and P < 0.03, respectively). However, other lipoprotein concentrations were unchanged. In conclusion, we found that endurance training induced significant elevation in plasma HDL-C and HDL2-C concentrations, accompanied by higher plasma Apo A-1, pre-beta HDL-C concentrations, LCAT activity and ABCA1 mRNA expressions in rat intestine, and liver.