Translation/secretion coupling by type III secretion systems
Translation/secretion coupling by type III secretion systems
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DOI：
10.1016/s0092-8674(00)00053-2
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发表时间：
2000-08-18
期刊：
影响因子：
64.5
通讯作者：
Hughes, KT
中科院分区：
文献类型：
--
作者：
Karlinsey, JE;Lonner, J;Hughes, KT
III型分泌系统介导革兰氏阴性菌中毒力蛋白和鞭毛组装亚基的输出。据信对每类分泌蛋白特异性的伴侣蛋白防止分泌底物的降解。我们发现，伴侣的一个额外的作用可能是调节分泌蛋白的翻译。我们表明，伴侣FlgN是必需的flgM基因转录从一个mRNA转录本（鞭毛3类转录本），但不是从另一个（鞭毛2类转录本）的翻译。从3类转录物翻译的FlgM主要被分泌，而从2类转录物翻译的FlgM主要保留在细胞质中。这些结果表明FlgM和其他III型分泌底物具有mRNA和氨基酸分泌信号，并支持III型伴侣蛋白在翻译/分泌偶联中的新作用。
Type III secretion systems mediate export of virulence proteins and flagellar assembly subunits in Gram-negative bacteria. Chaperones specific to each class of secreted protein are believed to prevent degradation of the secreted substrates. We show that an additional role of chaperones may be to regulate translation of secreted proteins. We show that the chaperone FlgN is required for translation of the flgM gene transcribed from one mRNA transcript (a flagellar class 3 transcript), but not from another (a flagellar class 2 transcript). FlgM translated from the class 3 transcript is primarily secreted whereas FlgM translated from the class 2 transcript is primarily retained in the cytoplasm. These results suggest FlgM and other type III secretion substrates possess both mRNA and amino acid secretion signals, and supports a new role for type III chaperones in translation/secretion coupling.