Rational use of the PFA-100 device for screening of platelet function disorders and von Willebrand disease
Rational use of the PFA-100 device for screening of platelet function disorders and von Willebrand disease
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DOI：
10.1097/00001721-200206000-00011
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发表时间：
2002-06-01
影响因子：
1.1
通讯作者：
Kirazli, S
中科院分区：
文献类型：
--
作者：
Buyukasik, Y;Karakus, S;Kirazli, S
用PFA-100仪器检测了225例接受血小板功能评估的患者和126名健康对照者。胶原/肾上腺素（CEPI）闭合时间（CT）的临界值为150秒，这是筛查血小板功能障碍和血管性血友病（vWD）的最可接受的敏感性（90%）、特异性（85.2%）、阳性（82.6%）和阴性（91.6%）预测值。所有vWD和Glanzmann血栓缺血症患者均可通过PFA-100检测。所有这些病例的CEPI和胶原/二磷酸腺苷（CADP） ct均升高。该装置对血小板分泌缺陷患者的灵敏度为81.6%。该亚组63.9%的患者CADP CT正常。在血红蛋白浓度较低的患者中，该仪器的特异性（47%）和阳性预测性（57%）降低。根据结果，开发了一种算法，用于筛选血小板功能障碍和vWD与PFA-100。
Two hundred and five patients referred for evaluation of platelet functions and 126 healthy controls were tested with the PFA-100 instrument. A cut-off value of 150 s for collagen/epinephrine (CEPI) closure time (CT) produced most acceptable sensitivity (90%), specificity (85.2%), and positive (82.6%) and negative (91.6%) predictivity values for screening of platelet function disorders and von Willebrand disease (vWD). All patients with vWD and Glanzmann thrombasthenia could be detected by PFA-100. Both CEPI and collagen/adenosine diphosphate (CADP) CTs were elevated in all of these cases. Sensitivity of the device was 81.6% for patients with platelet secretion defects. CADP CT was normal in 63.9% of the patients in this subgroup. Specificity (47%) and positive predictivity (57%) of the instrument were diminished in patients with low hemoglobin concentrations. Depending on the results, an algorithm was developed for screening of platelet function disorders and vWD with PFA-100.