HETEROGENEITY OF RIBOSOMAL GENES IN MICE AND MEN
HETEROGENEITY OF RIBOSOMAL GENES IN MICE AND MEN
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DOI：
10.1016/0092-8674(77)90053-8
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发表时间：
1977-01-01
期刊：
影响因子：
64.5
通讯作者：
SOUTHERN, EM
中科院分区：
文献类型：
--
作者：
ARNHEIM, N;SOUTHERN, EM
使用限制性内切酶Eco R1和Hind III研究了小鼠和人类核糖体DNA的结构。个体小鼠或人类显示出由非转录间隔区DNA的差异引起的限制性片段的异质性模式。来自近交系CBA-H-T6的6只个体小鼠具有相同的模式。另一种CBA菌株和C3 H显示了相同的模式。这些菌株最初来源于BALB. 1920年制造的DBA十字架。BALB/c、C57 BL和小家鼠的免疫表型不同。来自C3 H小鼠的培养细胞（L细胞）显示出与亲本株定量不同的模式，但来自BALB/c的2个骨髓瘤细胞系显示出与BALB/c小鼠相同的模式。人类的核糖体DNA也是异质的。在人类DNA之间观察到不同间隔区类别的量的差异。对小鼠-人细胞杂交的研究表明，一些间隔区类存在于5种人类核仁组织者中的一种以上。
The structures of mouse and human ribosomal DNA were studied using the restriction enzymes Eco R1 and Hind III. Individual mice or humans showed a heterogeneous pattern of restriction fragments resulting from differences in the non-transcribed spacer DNA. Six individual mice from the inbred strain CBA-H-T6 had identical patterns. The same pattern was shown by another CBA strain and by C3H. These strains were originally derived from a BALB .times. DBA cross made in 1920. Different patterns were found for BALB/c, C57BL and Mus poschiavinus. Cultured cells derived from C3H mice (L cells) showed a pattern quantitatively different from that of the parent strain, but 2 myeloma cell lines derived from BALB/c showed the same pattern as BALB/c mice. Ribosomal DNA in man is also heterogeneous. Differences were observed between human DNA in the amounts of the different spacer classes. Studies on mouse-human cell hybrids suggest that some spacer classes are present on more than one of the 5 human nucleolus organizers.