Soluble cytochrome b-c1 complex and the reconstitution of succinate-cytochrome c reductase.
Soluble cytochrome b-c1 complex and the reconstitution of succinate-cytochrome c reductase.
复制标题
可溶性细胞色素 b-c1 复合物和琥珀酸-细胞色素 c 还原酶的重建。
DOI：
10.1016/s0021-9258(19)42407-1
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
T. King
中科院分区：
文献类型：
--
作者：
C. Yu;L. Yu;T. King
The cytochromeb-c1complex has been “solubilized” and purified to a stage containing 6.5 nmoles of cytochromebper mg of protein. Seven bands are resolved on a polyacrylamide gel electrophoretic column in sodium dodecyl sulfateβ-mercaptoethanol medium. Five of these seven bands have been identified as cytochromesbandc1and a non-heme iron protein. The remaining two bands might be associated with these components or, less likely, might be impurities but do not belong to the so-called structural proteins. The cytochromeb-c1complex is enzymatically active and can reconstitute with soluble succinate dehydrogenase to form an integral entity of antimycin A-sensitive succinate-cytochromecreductase. The reconstituted reductase shows the same structural and functional characteristics as the intact reductase. The total number of thep-hydroxymercuribenzoate (p-MB) titratable groups in the cytochromeb-c1complex has been found to be 11 ± 1 moles per mole of cytochromeb. The p-MB-reacted complex is inactive in reconstitution but shows the same catalytic activity in the oxidation of reduced ubiquinone by cytochromec.
