VMAT-Mediated changes in quantal size and vesicular volume
VMAT-Mediated changes in quantal size and vesicular volume
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DOI：
10.1523/jneurosci.20-14-05276.2000
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发表时间：
2000-07-15
影响因子：
5.3
通讯作者：
Ewing, AG
中科院分区：
文献类型：
--
作者：
Colliver, TL;Pyott, SJ;Ewing, AG
已经确定分泌囊泡的体积可以被调节。然而，我们提出的第一个数据表明，在一个囊泡中的发射量可以调节其体积。用安培法和透射电镜观察了L-3，4-二羟基苯丙氨酸和利血平分别增加和减少嗜铬细胞瘤细胞单个囊泡的体积及其儿茶酚胺含量。由于囊泡中的儿茶酚胺含量的变化跟踪囊泡体积的变化，我们的研究结果表明，当量子大小通过囊泡单胺转运蛋白改变囊泡内的儿茶酚胺浓度保持相对恒定。这种以前未鉴定的细胞反应提供了新的见解，如何儿茶酚胺可以包装和分泌囊泡释放。
It has been well established that the volume of secretory vesicles can be modulated. However, we present the first data demonstrating that the amount of transmitter in a vesicle can regulate its volume. Amperometry and transmission electron microscopy have been used to determine that L-3,4-dihydroxyphenylalanine and reserpine increase and decrease, respectively, the volume of single pheochromocytoma cell vesicles as well as their catecholamine content. Because changes in vesicular catecholamine content are tracked by changes in vesicle volume, our results indicate that when quantal size is altered via the vesicular monoamine transporter the concentration of catecholamines within the vesicles remains relatively constant. This previously unidentified cellular response provides new insight into how catecholamines can be packaged in and released from secretory vesicles.