Axon terminals containing PACAP- and VIP-immunoreactivity form synapses with CRF-immunoreactive neurons in the dorsolateral division of the bed nucleus of the stria terminalis in the rat
Axon terminals containing PACAP- and VIP-immunoreactivity form synapses with CRF-immunoreactive neurons in the dorsolateral division of the bed nucleus of the stria terminalis in the rat
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DOI：
10.1016/s0006-8993(97)00737-3
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发表时间：
1997-08-29
期刊：
影响因子：
2.9
通讯作者：
Arimura, A
中科院分区：
文献类型：
--
作者：
Kozicz, T;Vigh, S;Arimura, A
终纹床核（BST）是一个高度异质性的前脑结构，其中中间和外侧BST发挥不同的功能作用。内侧BST（BSTM）被认为与性行为有关，而外侧BST（BSTL）可能具有与压力相关的功能。在人脑中，BST在血管活性肠多肽（VIP）免疫反应纤维的分布中显示出明显的性二态性，并且还含有非常高浓度的垂体腺苷酸环化酶激活多肽（PACAP）免疫反应性（ir）。用免疫组织化学方法观察大鼠脑内VIP和PACAP的传入纤维，发现它们在背外侧区（BSTLd）内分布丰富，而在背外侧区（BSTM）内不分布。IHC未显示两种性别之间在任一免疫反应性的分布大小方面存在任何明显差异。免疫组化双染显示VIP和PACAP神经元的轴突终末与促肾上腺皮质激素释放因子（CRF）神经元的树突或胞体紧密相连。在电镜水平上，免疫组化显示VIP-IR和PACAP-IR轴突终末与CRF-IR神经元之间存在轴树或轴体突触。虽然起源的PACAP-IR纤维在BSTLd仍有待确定，这些形态学的研究结果表明，PACAP和VIP调节CRF神经元的活动在BSTLd作为神经递质或神经调质。(C)1997年Elsevier Science B.V.
The bed nucleus of the stria terminalis (BST) is a highly heterogeneous forebrain structure, within which the median and lateral BST play distinct functional roles. The medial BST (BSTM) is thought to be related to sexual behavior, while the lateral BST (BSTL) may have a stress-related function. In the human brain, the BST shows marked sexual dimorphism in the distribution of vasoactive intestinal polypeptide (VIP) immunoreactive fibers and also contains a very high concentration of pituitary adenylate cyclase activating polypeptide (PACAP) immunoreactivity (ir). Using immunohistochemistry (IHC) to examine the rat brain, the present study found that both VIP and PACAP containing afferent fibers are abundant in the BSTLd (dorsolateral division of BST), but not in the BSTM. IHC did not reveal any apparent difference between the sexes in the size of distribution of either immunoreactivity. Double staining IHC showed that axonal terminals of both VIP and PACAP neurons were in close proximity to dendrites or perikarya of corticotropin releasing factor (CRF) neurons. At the electron microscopic level IHC revealed the presence of axodendritic or axosomatic synapses between VIP-ir and PACAP-ir axon terminals and CRF-ir neurons. Although the origin of PACAP-ir fibers in the BSTLd remains to be determined, these morphological findings suggest that PACAP and VIP regulate the activity of CRF neurons in the BSTLd as neurotransmitters or neuromodulators. (C) 1997 Elsevier Science B.V.