CHARACTERIZATION OF A TRIPLE OPIOID SYSTEM IN THE HUMAN NEUROBLASTOMA NMB CELL-LINE
CHARACTERIZATION OF A TRIPLE OPIOID SYSTEM IN THE HUMAN NEUROBLASTOMA NMB CELL-LINE
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DOI：
10.1016/0006-8993(94)91156-8
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发表时间：
1994-11-28
期刊：
影响因子：
2.9
通讯作者：
SARNE, Y
中科院分区：
文献类型：
--
作者：
BAUMHAKER, Y;BENDOR, T;SARNE, Y
人神经母细胞瘤NMB细胞株含有3种阿片受体(60%Delta、25%kappa和15%Mu)。阿片受体与腺苷环化酶负偶联，通过选择性激活单一受体类型最大限度地降低cAMP含量，表明多种阿片受体在同一细胞中共存。NMB细胞中的阿片受体在长期暴露于阿片拮抗剂纳洛酮后上调，在阿片激动剂依托托啡长期治疗后下调。下调具有时间、剂量和温度依赖性，可被秋水仙碱和叠氮化钠抑制。NMB培养被认为是研究不同阿片受体类型在同一神经元中共表达时的选择性激活和调节以及研究不同阿片受体之间相互作用的一个很好的实验模型。
The human neuroblastoma NMB cell line was found to contain the three types of opioid receptors (60% delta 25% kappa and 15% mu). The opioid receptors were negatively coupled to adenylyl-cyclase, Maximal reduction in cAMP content was achieved by selectively activating single receptor types, indicating the co-presence of the various opioid receptors in the same cells. The opioid receptors in NMB cells were up-regulated following prolonged exposure to the opioid antagonist naloxone and down-regulated following chronic treatment with the opioid agonist etorphine. Down-regulation was time-, dose- and temperature-dependent and was inhibited by colchicine and sodium azide. The NMB culture is presented as an excellent experimental model for studying the selective activation and regulation of the different opioid receptor types when they are co-expressed in the same neuron, as well as for studying interactions between the various opioid receptors.