Defining the transcriptomic and proteomic profiles of rat ageing skeletal muscle by the use of a cDNA array, 2D-and Blue native-PAGE approach
Defining the transcriptomic and proteomic profiles of rat ageing skeletal muscle by the use of a cDNA array, 2D-and Blue native-PAGE approach
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DOI：
10.1016/j.jprot.2009.02.007
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发表时间：
2009-05-02
影响因子：
3.3
通讯作者：
Moreno, M.
中科院分区：
文献类型：
--
作者：
Lombardi, A.;Silvestri, E.;Moreno, M.
我们定义了转录组和蛋白质组的档案，大鼠老化骨骼肌使用组合的cDNA阵列，2D-和蓝色的天然聚丙烯酰胺凝胶电泳方法。这是允许获得的相关事件的概述，导致转录组/蛋白质组/线粒体蛋白质组的变化可能是老年骨骼肌的结构/代谢特征的基础。主要差异在于与能量代谢、线粒体途径、肌原纤维丝和解毒相关的基因/蛋白质。关于线粒体OXPHOS复合物的丰度以及它们的超分子组织和活性，老年大鼠的线粒体，与年轻大鼠相比，含有显着较低量的复合物I（NADH：泛醌氧化还原酶），V（FoF 1-ATP合酶），和III（泛醇：细胞色素c氧化还原酶）。相同的线粒体含有大量的复合物II（琥珀酸;泛醌氧化还原酶），但复合物IV（细胞色素c氧化酶，考克斯）的量不变。当比较年轻和年老的肌肉线粒体之间的超复合物的配置文件，密度分析显示，较轻的超复合物显着减少，在老年人的线粒体，在老年组的主要超复合物带是那些代表较重的超复合物，可能表明一种补偿机制，在老化的肌肉，功能上是针对底物通道和催化增强呼吸体。(C)2009爱思唯尔有限公司版权所有。
We defined the transcriptomic and proteomic profiles of rat ageing skeletal muscle using a combined cDNA array, 2D- and Blue native-PAGE approach. This was allowed to obtain an overview of the interrelated events leading to the transcriptome/proteome/mitoproteome changes likely to underlie the structural/metabolic features of aged skeletal muscle. The main differences were found in genes/proteins related to energy metabolism, mitochondrial pathways, myofibrillar filaments, and detoxification. Concerning the abundance of mitochondrial OXPHOS complexes as well as their supramolecular organization and activity, mitochondria from old rats, when compared with those from young rats, contained significantly lower amounts of complex I (NADH:ubiquinone oxidoreductase), V (FoF1-ATP synthase), and III (ubiquinol:cytochrome c oxidoreductase). The same mitochondria contained a significantly larger amount of complex II (succinate;ubiquinone oxidoreductase), but an unchanged amount of complex IV (cytochrome c oxidase, COX). When comparing the supercomplex profiles between young and old muscle mitochondria, the densitometric analysis revealed that lighter supercomplexes were significantly reduced in older mitochondria, and that in the older group the major supercomplex bands were those representing heavier supercomplexes, likely suggesting a compensatory mechanism that, in ageing muscle, is functionally directed towards substrate channeling and catalytic enhancement advantaging the respirosome. (C) 2009 Elsevier B.V. All rights reserved.