Langerhans cells: functional aspects revealed by in vivo grafting studies.
Langerhans cells: functional aspects revealed by in vivo grafting studies.
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朗格汉斯细胞：体内移植研究揭示的功能方面。
DOI：
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发表时间：
1980
影响因子：
6.5
通讯作者：
P. Bergstresser
中科院分区：
文献类型：
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作者：
J. Streilein;G. Toews;P. Bergstresser
我们进行了两种类型的实验来揭示朗格汉斯细胞功能的各个方面。在第一种类型中，将尾皮（小鼠）和颊囊（仓鼠）异位移植到正常受体的胸壁，随后涂上免疫剂量的化学接触剂二硝基氟苯。在第二种类型中，将用紫外线照射的皮质组织和皮肤移植到I类（小鼠K/D）或II类（Ia）抗原免疫遗传学不同的受体上，以确定这些移植物引起同种异体免疫的能力。我们发现，完整的小鼠尾部皮肤不能支持接触敏感性的诱导是皮肤本身的属性，而不是解剖部位。无法通过检查囊致敏是囊的解剖学布置的特性，因为不存在有效的淋巴引流途径。虽然紫外线明显地耗尽了体壁皮肤的ATP酶阳性细胞，但它未能以高度免疫原性的方式消除皮肤表达Ia抗原的能力。角膜移植物与其宿主的主要组织相容性复合体的I区不同，既不能引起自身的排斥反应，也不能损害宿主对携带相同I区抗原的体壁皮肤同种异体移植物的后续反应。这些结果是有力的旁证，朗格汉斯细胞是重要的表皮因子，促进诱导接触性过敏。紫外线似乎是暂时扰乱朗格汉斯细胞功能的有效方式，但不是从皮肤中去除朗格汉斯细胞的有效手段。角膜移植实验的结果提供了希望，即当发现从皮肤上清除朗格汉斯细胞的有效方法时，皮肤将缺乏其促进接触性超敏反应和引起针对Ia抗原的同种异体免疫的能力。
We performed 2 types of experiments to reveal aspects of Langerhans cell function, In the 1st type, tail skins (mice) and cheek pouches (hamsters) were grafted heterotopically to the thoracic wall of normal recipients and were subsequently painted with immunizing doses of a chemical contactant, dinitrofluorobenzene. In the 2nd type, cortical tissue and skinirradiated with ultraviolet light were grafted to recipients immunogenetically disparate for class I (murine K/D) or class II (Ia) antigens to determine the ability of these grafts to elicit allograft immunity. We found that the inability of intact murine tail skin to support the induction of contact sensitivity was a property of the skin itself, not of the anatomical site. The inability to sensitize through check pouch was a property of the anatomical arrangement of the pouch in that an effective lymphatic drainage pathway did not exist. Although ultraviolet light apparently depleted body wall skin of ATPase-positive cells, it failed to rid skin of Its capacity to express Ia antigens in a highly immunogenic ways Cornea grafts differing from their hosts across the I region alone of the major histocompatibility complex succeeded neither in inciting their own rejection nor in prejudicing the host's subsequent response to body wall skin allografts bearing the same I region antigens. These results are strong circumstantial evidence that Langerhans cells are the important epidermal factors promoting induction of contact hyper-sensitivity. Ultraviolet light appears to be an effective way in which to transiently perturb Langerhans cell function, but is not an effective means of removing Langerhans cells from skin. The results of the cornea graft experiments offer hope that, when effective means of erasing Langerhans cells from skin have been found, skin will be devoid of its capacity to promote contact hypersensitivity and to elicit allograft immunity directed at Ia antigens.