IMMUNOLOGICAL AND CLINICAL SPECIFICITY OF THE IMMUNOCHEMICAL DETERMINATION OF PROSTATIC ACID PHOSPHATASE
IMMUNOLOGICAL AND CLINICAL SPECIFICITY OF THE IMMUNOCHEMICAL DETERMINATION OF PROSTATIC ACID PHOSPHATASE
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前列腺酸性磷酸酶免疫化学测定的免疫学和临床特异性
DOI：
10.1111/j.1749-6632.1982.tb40305.x
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发表时间：
1982
影响因子：
5.2
通讯作者：
William Croop
中科院分区：
文献类型：
--
作者：
L. Shaw;N. Yang;M. Neat;William Croop
我们一直对研究免疫化学方法测定患者血清中前列腺酸性磷酸酶水平的免疫学和临床特异性感兴趣。我们对此感兴趣的原因是：(1) 与催化测定相比，免疫化学方法有望为前列腺酸性磷酸酶的测量提供更高的特异性。没有任何用于测定催化酸性磷酸酶活性的底物已被证明对酸性磷酸酶的前列腺同工酶具有绝对特异性。 (2) 由于我们临床实验室有责任与医生合作解释患者血清中前列腺酸性磷酸酶的异常水平，因此我们需要严格定义免疫反应性前列腺酸性磷酸酶升高的所有可能来源。为此，我们利用免疫过氧化物酶技术研究了（a）免疫反应性前列腺酸性磷酸酶在正常和患病人体组织中的分布； (b)通过放射免疫测定技术，来自非前列腺组织的酸性磷酸酶的几种纯化制剂与抗前列腺酸性磷酸酶抗血清的交叉反应性； (c) 非前列腺组织和血细胞的提取物以及妇女的血清和尿液中可能存在免疫反应性前列腺酸性磷酸酶； [d]未患有前列腺腺癌的男性患者血清中免疫反应性前列腺酸性磷酸酶的定量水平，以确定该程序的临床特异性。
We have been interested in studying the immunological and clinical specificity of immunochemical methods for the determination of prostatic acid phosphatase levels in patients’ sera. Our reasons for this interest are: (1) Immunochemical methods offer the promise of providing greater specificity for the measurement of prostatic acid phosphatase compared to catalytic assays. No substrate for the determination of catalytic acid phosphatase activity has been shown to be absolutely specific for the prostatic isoenzyme of acid phosphatase. (2) Since we in the clinical laboratory have the responsibility to interpret abnormal levels of prostatic acid phosphatase in patients’ sera in collaboration with physician staff, we need to define rigorously all possible sources of elevated immunoreactive prostatic acid phosphatase. To this end we have studied (a) the distribution of immunoreactive prostatic acid phosphatase in normal and diseased human tissues with an immunoperoxidase technique; (b) the cross-reactivity of several purified preparations of acid phosphatase from nonprostatic tissues with antiprostatic acid phosphatase antiserum by a radioimmunoassay technique; (c) the possible presence of immunoreactive prostatic acid phosphatase in extracts of nonprostatic tissues and blood cells, and in the serum and urine of women; and [d] the quantitative levels of immunoreactive prostatic acid phosphatase in the serum of male patients who do not have adenocarcinoma of the prostate, in order to determine the clinical specificity of this procedure.
人肝酸性磷酸酶：低分子量同工酶的纯化和特性。
影响因子：
3.9
作者：
Taga,EM;VanEtten,RL
通讯作者：
VanEtten,RL