Lead Inhibits Human Sperm Functions by Reducing the Levels of Intracellular Calcium, cAMP, and Tyrosine Phosphorylation
Lead Inhibits Human Sperm Functions by Reducing the Levels of Intracellular Calcium, cAMP, and Tyrosine Phosphorylation
复制标题
铅通过降低细胞内钙、cAMP 和酪氨酸磷酸化水平来抑制人类精子功能
DOI：
10.1620/tjem.238.295
复制
发表时间：
2016-04-01
影响因子：
2.2
通讯作者：
Zeng, Xuhui
中科院分区：
文献类型：
--
作者：
He, Yuanqiao;Zou, Qianxing;Zeng, Xuhui
众所周知，近年来，全球范围内男性生育能力不断下降。影响这一现象的主要因素之一是环境污染。铅是威胁我国动物和人类健康的主要重金属污染物之一。它优先积聚在男性生殖器官中，在人精浆中可达10μM。铅会损害哺乳动物体内的精子发生和精子质量。它还在体外抑制精子功能，但其潜在机制仍不清楚。因此，我们的目的是研究铅对人类精子功能的体外毒性并阐明其潜在机制。精液样本采集自居住在江西南昌市的20名不同职业和背景的健康志愿者。用不同浓度的醋酸铅（0、0.5、2.5、10、50和100μM）处理人精子悬浮液，并检查活力、运动性、获能和孕酮诱导的顶体反应。用10-100μM醋酸铅处理剂量依赖性地抑制总的和渐进的运动测量、获能和黄体酮诱导的顶体反应。它还以剂量依赖性方式降低细胞内环磷酸腺苷 (cAMP) 和钙 ([Ca2+](i)) 的浓度，并降低精子蛋白的酪氨酸磷酸化，所有这些都被认为是调节精子功能的关键因素。我们的研究结果表明，铅通过降低精子细胞内 cAMP [Ca2+](i) 的水平来抑制人类精子功能；和体外精子蛋白酪氨酸磷酸化。
It is well known that there has been a worldwide decrease in human male fertility in recent years. One of the main factors affecting this is environmental pollution. Lead is one of the major heavy metal contaminants that threaten the health of animals and human beings in China. It preferentially accumulates in male reproductive organs and can be up to 10 mu M in human seminal plasma. Lead impairs mammalian spermatogenesis and sperm quality in vivo. It also inhibits sperm functions in vitro but the underlying mechanisms remain unclear. Therefore, we aimed to investigate the in vitro toxicity of lead on human sperm functions and to elucidate the underlying mechanisms. Semen samples were collected from 20 healthy volunteers with different careers and backgrounds living in Nanchang, Jiangxi. Human sperm suspensions were treated with different concentrations of lead acetate (0, 0.5, 2.5, 10, 50, and 100 mu M) and the viability, motility, capacitation and progesterone -induced acrosome reaction were examined. Treatment with 10-100 mu M lead acetate dose-dependently inhibited total and progressive motility measures, capacitation and progesterone -induced acrosome reaction. It also dose-dependently decreased the intracellular concentrations of cyclic adenosine monophosphate (cAMP) and calcium ([Ca2+](i)), and reduced the tyrosine phosphorylation of sperm proteins, all of which are thought to be key factors in the regulation of sperm function. Our findings suggest that lead inhibits human sperm functions by reducing the levels of sperm intracellular cAMP, [Ca2+](i); and tyrosine phosphorylation of sperm proteins in vitro.