Sodium acetate decreases phosphorylation of hormone sensitive lipase in isoproterenol-stimulated 3T3-L1 mature adipocytes
Sodium acetate decreases phosphorylation of hormone sensitive lipase in isoproterenol-stimulated 3T3-L1 mature adipocytes
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DOI：
10.4161/adip.27936
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发表时间：
2014-01-01
期刊：
影响因子：
3.3
通讯作者：
Ball, Derek
中科院分区：
文献类型：
--
作者：
Aberdein, Nicola;Schweizer, Michael;Ball, Derek
脂肪分解是将储存的三酰甘油水解为甘油和非酯化脂肪酸(NEF A)的过程，据报道可被短链脂肪酸(SCFA)还原，但这种抑制的机制尚不清楚。这项研究的目的是测量激素敏感脂肪酶在醋酸钠暴露和不暴露的情况下丝氨酸残基563的磷酸化。在3T3-L1细胞系中，我们发现与基础状态相比，异丙肾上腺素刺激细胞后，磷酸化激素敏感脂肪酶(PHSL)的表达增加了60%。在SCFA醋酸酯存在的刺激细胞中，PHSL比单独刺激细胞下降了15%。醋酸钠处理60min和180min后，NEFA的释放量分别由0.53mU·mg~(-1)蛋白和1.13mU·mg~(-1)蛋白降低到0.41mU·mg~(-1)蛋白(P=0.004)和1.73mU·mg~(-1)蛋白(P=0.020)，但对甘油释放量无明显影响(P=0.109)。总之，暴露于4 mM醋酸盐可降低成熟3T3-L1脂肪细胞中HSL(SER563)的磷酸化水平，并导致NEF A的释放显著减少，尽管甘油的释放没有受到影响。
Lipolysis, the process of hydrolysis of stored triacylglycerol into glycerol and non-esterified fatty acids (NEF A), is reported to be reduced by short chain fatty acids (SCFA) but the mechanism of this inhibition is poorly understood. The aim of this study was to measure the phosphorylation at serine residue 563 of hormone sensitive lipase with and without exposure to sodium acetate. Using the 3T3-L1 cell line, we identified that stimulating the cells with isoproterenol increased phosphorylated hormone sensitive lipase (pHSL) expression by 60% compared with the basal state. In the presence of the SCFA acetate in stimulated cells, pHSL decreased by 15% compared with stimulated cells alone. These results were mirrored by the NEF A release from stimulated cells that had significantly decreased in the presence of sodium acetate after 60 min (from 0.53 mu mol mg(-1) protein to 0.41 mu mol mg(-1) protein, respectively, P = 0.004); and 180 min (1.73 mu mol mg(-1) protein to 1.13 mu mol mg(-1) protein, P = 0.020); however, treatment had no effect on glycerol release (P = 0.109). In conclusion, exposure to 4 mM acetate reduced the level of phosphorylation of HSL(SER563) in mature 3T3-L1 adipocytes and led to a significant reduction in NEF A release, although glycerol release was not affected.