Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry
Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry
复制标题
DOI：
10.1016/j.jchromb.2005.10.006
复制
发表时间：
2005-12-27
影响因子：
3
通讯作者：
Deelder, AM
中科院分区：
文献类型：
--
作者：
Bruggink, C;Wuhrer, M;Deelder, AM
建立了毛细管高pH阴离子交换色谱（HPAEC）分析糖类化合物的方法，并结合了两种亚皮摩尔灵敏度的在线检测方法：（1）脉冲安培检测（PAD）;（2）毛细管电泳-电喷雾离子阱（IT）质谱（MS）。毛细管色谱系统结合了高超的选择性HPAEC，允许常规分离的异构体寡糖与信息的单糖序列和连接位置获得的MS/MS碎片使用IT-MS。该系统在生物医学研究中的适用性证明了其用于G（M1）-神经节苷脂病患者的尿样分析。样品中的异构体聚糖可以通过HPAEC解析，并根据在线IT-MS/MS显示的单糖键合信息进行归属。（c）2005 Elsevier B. V.保留所有权利。
A capillary-scale high-pH anion-exchange chromatography (HPAEC) system for the analysis of carbohydrates was developed, in combination with two parallel on-line detection methods of sub-picomolar sensitivity: (1) pulsed amperometric detection (PAD); (2) capillary-scale desalting followed by electrospray ion-trap (IT) mass spectrometry (MS). The capillary chromatographic system combined the superb selectivity of HPAEC that allows routine separation of isomeric oligosaccharides with the information on monosaccharide sequence and linkage positions obtained by MS/MS fragmentation using the IT-MS. The applicability of the system in biomedical research was demonstrated by its use for the analysis of a urine sample of a G(M1)-gangliosidosis patient. Isomeric glycans in the sample could be resolved by HPAEC and assigned on the basis of the monosaccharide linkage information revealed by on-line IT-MS/MS. (c) 2005 Elsevier B.V. All rights reserved.