Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry.
Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry.
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DOI：
10.1007/978-1-0716-1998-8_13
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发表时间：
2022
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Metabolomics is the systems-scale study of the biochemical intermediates of metabolism. This approach has great potential to uncover how metabolic intermediates are used and generated in cell-free expression systems, something that is to date not well understood. Here, we present a detailed metabolomics protocol for characterization of the small molecules in cell-free systems. We specifically focus on the analysis of Escherichia coli lysate-based cell-free systems using gas chromatography coupled to mass spectrometry. Measuring and monitoring the metabolic changes in cell-free systems can provide insight into the ways that metabolites affect the productivity of cell-free reactions, ultimately allowing for more informed engineering and optimization efforts for cell-free systems.
