Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil
Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil
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DOI：
10.1007/s10482-010-9438-0
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发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Koh, Chong-Lek
中科院分区：
文献类型：
--
作者：
Chan, Kok-Gan;Wong, Cheng-Siang;Koh, Chong-Lek
从马来西亚热带雨林土壤样品中分离出一株菌株KM1S，该菌株使用特定的富集培养基，特别有利于群体猝灭细菌的选择。通过16S核糖体DNA序列分析，KM1S与蜡样芽孢杆菌聚类密切。通过快速分辨液相色谱法测定，n -3-氧-己烯基同型丝氨酸内酯和n -3-氧-辛烷基同型丝氨酸内酯在体外降解速度分别为4.98和6.56 μ g AHL h(-1) / 10(9) CFU/ml。扩增并克隆了KM1S的aiiA同源物，该同源物编码催化n -酰基高丝氨酸内酯降解的自诱导剂失活酶。序列分析表明，n -酰基高丝氨酸内酯内酯酶存在HXDH-59 - h (169)-21 - d（191）基序（106）。
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 mu g AHL h(-1) per 10(9) CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif (106)HXDH-59 amino acids-H(169)-21 amino acids-D(191) for N-acylhomoserine lactone lactonases.