Immunohistochemical study of osteopontin and L-selectin in a rat endometriosis model and in human endometriosis
Immunohistochemical study of osteopontin and L-selectin in a rat endometriosis model and in human endometriosis
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DOI：
10.1016/j.fertnstert.2007.02.007
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发表时间：
2007-10-01
影响因子：
6.7
通讯作者：
Suzuki, Mitsuaki
中科院分区：
文献类型：
--
作者：
Odagiri, Kohei;Konno, Ryo;Suzuki, Mitsuaki
目的：研究骨桥蛋白（OPN）和L-选择素（SELL）在人胚肾组织中的定位。设计图：回顾性非随机免疫组化研究在手术诱导的腹膜子宫内膜异位症大鼠模型和人类卵巢子宫内膜异位病变样本。单位：一所大学医院的妇科。患者：通过手术自体移植子宫，在10只8周龄SLC-Sprague-道利大鼠中诱导大鼠子宫内膜异位症模型。14名绝经前妇女组织学诊断子宫内膜异位症（平均年龄，39岁;范围，25-53岁）。从14例接受腹腔镜手术治疗扩大的卵巢囊肿的患者中获得20个卵巢囊肿标本。干预（S）：通过免疫组化对卵巢增生标本进行OPN和SELL表达的组织学检查。主要结局指标：大鼠子宫内膜异位症模型和人子宫内膜异位症组织中OPN和SELL表达的免疫反应性染色的证明。结果：在来自大鼠子宫内膜异位症模型和来自人子宫内膜异位症的两种组织中，OPN在腺上皮中比在间质空间中更显著地染色，而SELL在间质空间（巨噬细胞和淋巴细胞）中比在上皮中更显著地染色。OPN在异位子宫内膜异位症中的染色模式与分泌期在位正常人子宫内膜中的染色模式非常相似。结论：OPN和SELL在子宫内膜异位症的发病中起重要作用。（Fertil Steril（R）2007;88（增刊2）：1207-11.（C）2007年由美国生殖医学协会。
Objective: To investigate the localization of the proteins osteopontin (OPN) and L-selectin (SELL). Design: Retrospective nonrandomized immunohistochemical study in a surgically induced rat model of peritoneal endometriosis and in samples of human endometriotic lesions of ovaries. Setting: Department of gynecology in a university hospital. Patient(s): A rat endometriosis model was induced in 10 8-week-old SLC-Sprague-Dawley rats by surgical autotransplantation of uterus. Fourteen premenopausal women with histologically diagnosed endometriosis were selected (mean age, 39 y; range, 25-53 y). Twenty endometriotic specimens were obtained from 14 patients who underwent laparoscopic surgery for enlarged endometriotic cysts. Intervention(S): Histopathological examination of endometriotic ovarian specimens for OPN and SELL expression by immumohistochemistry. Main Outcome Measure(s): Demonstration of the immunoreactive staining of OPN and SELL expressions in tissues of a rat endometriosis model and of human endometriosis. Result(s): In both tissues from a rat endometriosis model and from human endometriosis, OPN was stained more prominently in glandular epithelium than in interstitial space, whereas SELL stained more prominently in interstitial space (macrophages and lymphocytes) than in epithelium. The staining pattern of OPN in ectopic endometriotic lesions was very similar to that in eutopic normal human endometrium in the secretory phase. Conclusion(S): These results suggested important roles for OPN and SELL in the pathogenesis of endometriosis. (Fertil Steril (R) 2007;88(Suppl 2):1207-11. (C)2007 by American Society for Reproductive Medicine.)