Determination of cDNA, gene structure and chromosomal localization of the novel human 17β-hydroxysteroid dehydrogenase type 7
Determination of cDNA, gene structure and chromosomal localization of the novel human 17β-hydroxysteroid dehydrogenase type 7
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DOI：
10.1016/s0014-5793(99)01366-6
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发表时间：
1999-10-29
期刊：
影响因子：
3.5
通讯作者：
Adamski, J
中科院分区：
文献类型：
--
作者：
Krazeisen, A;Breitling, R;Adamski, J
17 β-羟基类固醇脱氢酶7型（17 β-HSD 7）是一种新的人cDNA编码的37 kDa蛋白质，与大鼠和小鼠17 β-HSD 7的氨基酸同源性分别为78%和74%。这些酶负责妊娠期间黄体中雌二醇的产生，但也存在于胎盘和几种类固醇靶组织中人17 β-HSD 7基因（HSD 17 B7）由9个外显子和8个内含子组成，跨越21.8 kb，定位于染色体10 p11.2，靠近肿瘤进展的易感位点，肥胖和糖尿病。HSD 17 B7启动子（1.2 kb）揭示了脑特异性和淋巴样转录因子的结合位点，其对应于通过计算机北方印迹鉴定的造血组织和发育中的脑中的额外表达结构域。(C)1999年欧洲生物化学学会联合会。
nle have identified human 17 beta-hydroxysteroid dehydrogenase type 7 (17 beta-HSD 7), The novel human cDNA encodes a 37 kDa protein that shows 78 and 74% amino acid identity with rat and mouse 17 beta-HSD 7, respectively. These enzymes are responsible for estradiol production in the corpus luteum during pregnancy, but are also present in placenta and several steroid target tissues (breast, testis and prostate) as revealed by RT-PCR, The human 17 beta-HSD 7 gene (HSD17B7) consists of nine exons and eight introns, spanning 21.8 kb and maps to chromosome 10p11.2 close to susceptibility loci for tumor progression, obesity and diabetes. The HSD17B7 promoter (1.2 kb) reveals binding sites for brain-specific and lymphoid transcription factors corresponding to additional expression domains in hematopoietic tissues and the developing brain as identified by in silico Northern blot. (C) 1999 Federation of European Biochemical Societies.