Influence of surface energy distribution on neuritogenesis
Influence of surface energy distribution on neuritogenesis
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DOI：
10.1016/j.colsurfb.2009.04.006
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发表时间：
2009-09-01
影响因子：
5.8
通讯作者：
Hamraoui, Ahmed
中科院分区：
文献类型：
--
作者：
Lamour, Guillaume;Journiac, Nathalie;Hamraoui, Ahmed
PC12细胞是研究神经元分化的有用模型，因为它们通常在神经生长因子（NGF）的作用下可以进行终末分化。本研究利用原子力显微镜（AFM）和免疫荧光技术研究了表面能分布对PC12细胞分化的影响。玻璃表面通过化学吸附改性：氨基硅烷。用缩聚法接枝n-[3-（三甲氧基硅基）丙基]乙二胺（C8H22N2O3Si； EDA）。基质形貌的AFM分析表明，聚集体的存在表明吸附是不均匀的，并且在粘附能量上产生局部梯度。在没有NGF处理的情况下，在这些修饰过的玻璃表面培养的PC12细胞出现了神经突。相比之下，在没有NGF的情况下，PC12细胞在相对光滑的表面（如聚l -赖氨酸底物）上没有生长出神经突，其中胺的分布相当均匀。这些结果表明，通过细胞-底物相互作用，表面能量分布触发了驱动PC12细胞分化和启动神经新生的机制。我们能够创造一种受控的物理纳米结构，具有局部表面能变化，从而可以研究这些参数对神经生成的影响。(C) 2009 Elsevier B.V.版权所有
PC12 cells are a useful model to study neuronal differentiation, as they can undergo terminal differentiation, typically when treated with nerve growth factor (NGF). In this study we investigated the influence of surface energy distribution on PC12 cell differentiation, by atomic force microscopy (AFM) and immunofluorescence. Glass surfaces were modified by chemisorption: an aminosilane. n-[3-(trimethoxysilyl)propyl]ethylendiamine (C8H22N2O3Si; EDA), was grafted by polycondensation. AFM analysis of substrate topography showed the presence of aggregates suggesting that the adsorption is heterogeneous, and generates local gradients in energy of adhesion. PC12 cells cultured on these modified glass surfaces developed neurites in absence of NGF treatment. In contrast, PC12 cells did not grow neurites when cultured in the absence of NGF on a relatively smooth surface such as poly-L-lysine substrate, where amine distribution is rather homogeneous. These results suggest that surface energy distribution, through cell-substrate interactions, triggers mechanisms that will drive PC12 cells to differentiate and to initiate neuritogenesis. We were able to create a controlled physical nano-structuration with local variations in surface energy that allowed the study of these parameters on neuritogenesis. (C) 2009 Elsevier B.V. All rights reserved.