ECDYSTEROID TITER DURING LARVAL-PUPAL-ADULT DEVELOPMENT OF THE TOBACCO HORNWORM, MANDUCA-SEXTA
ECDYSTEROID TITER DURING LARVAL-PUPAL-ADULT DEVELOPMENT OF THE TOBACCO HORNWORM, MANDUCA-SEXTA
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DOI：
10.1016/0016-6480(81)90005-8
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
GILBERT, LI
中科院分区：
文献类型：
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作者：
BOLLENBACHER, WE;SMITH, SL;GILBERT, LI
在第4龄（第二龄）和第5龄（第5龄），卵外激素滴度分别在24 h和60 h左右大幅上升，并在蜕皮前达到峰值。在幼虫的最后阶段，还观察到一个较早的、较小的滴度增加，持续约20小时，这一增加被认为是对蛹发育的承诺。化蛹后，激素滴度再次升高，在第7-9天达到最大值。在化蛹后第10天，滴度急剧下降，稳定在0.0.1%。14天。在蛹-成虫发育过程中，体外激素滴度不存在两性二态性。在成年羽化后，观察到雄性和雌性之间表皮激素滴度的差异。放射免疫活性定性分析显示，根据发育阶段，仅存在蜕皮激素和20-羟基蜕皮激素，其比例不同。在幼虫和蛹蜕皮前的高峰，20-羟基蜕皮激素是主要激素；蜕皮激素是蛹-成虫发育过程中的主要成分。第5龄承诺峰由大约等量的2种蜕皮甾体组成。
The ecdysteroid titer during the 4th (penultimate) and 5th larval instars of M. sexta was characterized by large increases lasting approximately 24 and 60 h, respectively, and these peaks occurred just before ecdysis. During the last larval instar, an earlier, smaller increase in the titer also was observed lasting approximately 20 h and is the increase presumed responsible for commitment to pupal development. After pupation the ecdysteroid titer increases again, reaching a maximum between days 7-9. At day 10 postpupation the titer declined dramatically and stabilized at .apprx. day 14. During pupal-adult development there was no sexual dimorphism in the ecdysteroid titer. A difference in the ecdysteroid titer between males and females was observed after adult eclosion. Qualitative analysis of the ecdysteroid radioimmunoassay activity revealed the presence of only ecdysone and 20-hydroxyecdysone at varying ratios depending on developmental stage. For the large peaks preceding larval and pupal ecdysis, 20-hydroxyecdysone was the predominant hormone; ecdysone was the major component during pupal-adult development. The 5th instar commitment peak was composed of approximately equal quantities of the 2 ecdysteroids.