Single-step fabrication of polydimethylsiloxane microwell arrays with long-lasting hydrophilic inner surfaces
Single-step fabrication of polydimethylsiloxane microwell arrays with long-lasting hydrophilic inner surfaces
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DOI：
10.1063/1.5025076
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发表时间：
2018-05-21
影响因子：
4
通讯作者：
Taguchi, Mitsumasa
中科院分区：
文献类型：
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作者：
Oyama, Tomoko Gowa;Barba, Bin Jeremiah Duenas;Taguchi, Mitsumasa
我们提出了一种单步制造方法，用于聚二甲基硅氧烷（PDMS）细胞粘附性微孔阵列，其具有持久（在水介质中>10个月）亲水性内表面，而不需要任何化学处理，例如显影。在空气中用低能电子束（55 kV）照射PDMS膜在PDMS表面上产生类似于40 μ m厚的亲水性二氧化硅样层，这是延长亲水性的关键。此外，受照射地区的压实产生了几十微米深的凹威尔斯。在疏水性未辐照PDMS表面上产生的亲水性微孔容易捕获纳升/皮升液滴和细胞/单细胞。此外，微孔的表面提供了稳定和有利的细胞粘附环境。该方法可以实现稳定可靠的芯片实验室，满足生物和医学应用领域日益增长的需求。出版社：AIP Publishing
We propose a single-step fabrication method for polydimethylsiloxane (PDMS) cell-adhesive microwell arrays with long-lasting (>10 months in aqueous medium) hydrophilic inner surfaces without the need for any chemical treatment such as development. Irradiation of a PDMS film with a low-energy electron beam (55 kV) in air generated a similar to 40-mu m-thick hydrophilic silica-like layer on the PDMS surface, which was the key to the prolonged hydrophilicity. Moreover, the concomitant compaction of the irradiated area produced dozens-of-micrometers-deep concave wells. The hydrophilic microwells generated on the hydrophobic non-irradiated PDMS surface easily trapped nano-/picoliter droplets and cells/single-cells. In addition, the surfaces of the microwells offered stable and favorable cell-adherent environments. The method presented here can realize stable and reliable lab-on-chips and cater to the expanding demand in biological and medical applications. Published by AIP Publishing.