Effect of High Fat Diet Enriched With Unsaturated and Diet Rich in Saturated Fatty Acids on Sphingolipid Metabolism in Rat Skeletal Muscle
Effect of High Fat Diet Enriched With Unsaturated and Diet Rich in Saturated Fatty Acids on Sphingolipid Metabolism in Rat Skeletal Muscle
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DOI：
10.1002/jcp.22283
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发表时间：
2010-12-01
影响因子：
5.6
通讯作者：
Gorski, Jan
中科院分区：
文献类型：
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作者：
Blachnio-Zabielska, Agnieszka;Baranowski, Marcin;Gorski, Jan
高脂饮食导致肌肉脂质蓄积，这是参与诱导胰岛素抵抗的重要因素。神经酰胺可能部分抑制胰岛素信号级联反应。本研究旨在探讨不同高脂饮食对大鼠骨骼肌神经酰胺代谢的影响。对大鼠进行了5周的实验：（1）标准食物和（2）富含多不饱和脂肪酸（PUFA）的高脂肪饮食和（3）富含饱和脂肪酸（SAT）的饮食。对三种类型的肌肉进行测定：慢收缩氧化（比目鱼肌）、快收缩氧化糖酵解和快收缩糖酵解（分别为腓肠肌的红色和白色部分）。丝氨酸棕榈酰转移酶（SPT），中性和酸性鞘磷脂酶（n-和aSM酶），中性和碱性神经酰胺酶（n-和alCD酶）的活性进行了检查。神经酰胺，鞘氨醇-1-磷酸的含量也进行了测量。PUFA饮食组的任何肌肉中的神经酰胺含量没有变化，但SAT饮食组的比目鱼肌和腓肠肌红段的神经酰胺含量分别增加了46%和52%。SAT饮食组中神经酰胺含量升高可能是SPT活性增加和同时nCD酶活性降低的结果。PUFA组神经酰胺含量无明显变化可能是由于SPT和alCD酶活性增加而SM酶活性降低所致。我们的结论是肌肉神经酰胺水平的调节取决于饮食和骨骼肌的类型。J.细胞。225：786-791，2010。(C)2010 Wiley-Liss，Inc.
Consumption of high fat diet leads to muscle lipid accumulation which is an important factor involved in induction of insulin resistance. Ceramide is likely to partially inhibit insulin signaling cascade. The aim of this study was to examine the effect of different high fat diets on ceramide metabolism in rat skeletal muscles. The experiments were carried out on rats fed for 5 weeks: (1) a standard chow and (2) high fat diet rich in polyunsaturated fatty acids (PUFA) and (3) diet enriched with saturated fatty acids (SAT). Assays were performed on three types of muscles: slow-twitch oxidative (soleus), fast-twitch oxidative-glycolytic, and fast-twitch glycolytic (red and white section of the gastrocnemius, respectively). The activity of serine palmitoyltransferase (SPT), neutral and acid sphingomyelinase (n- and aSMase), and neutral and alkaline ceramidase (n- and alCDase) was examined. The content of ceramide, sphinganine, sphingosine, and sphingosine-1-phosphate was also measured. The ceramide content did not change in any muscle from PUFA diet group but increased in the SAT diet group by 46% and 52% in the soleus and red section of the gastrocnemius, respectively. Elevated ceramide content in the SAT diet group could be a result of increased SPT activity and simultaneously decreased activity of nCDase. Unchanged ceramide content in the PUFA diet group might be a result of increased activity of SPT and alCDase and simultaneously decreased activity of SMases. We conclude that regulation of muscle ceramide level depends on the diet and type of skeletal muscle. J. Cell. Physiol. 225: 786-791, 2010. (C) 2010 Wiley-Liss, Inc.