Synthesis and Conformation of Monodispersed Oligo(ε-L-Lysine)mers Studied by Cd and IR Spectroscopies
Synthesis and Conformation of Monodispersed Oligo(ε-L-Lysine)mers Studied by Cd and IR Spectroscopies
复制标题
通过 Cd 和红外光谱研究单分散寡聚 (ε-L-赖氨酸) 的合成和构象
DOI：
10.1080/00387019508010070
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hiroyuki Yamamoto
中科院分区：
文献类型：
--
作者：
Ho;H. Yamaguchi;Daibou Fujimori;T. Ohnishi;A. Nishida;Hiroyuki Yamamoto
摘要 采用液相法合成了一系列单分散寡聚（e-L-赖氨酸）（2-、4-、6-和 8-聚体），它们提供了微生物产生的聚（e-L-赖氨酸）（e-PLL）的片段结构。利用圆二色性和红外光谱研究了寡肽的构象，并与e-PLL的结果进行了比较。 CD 光谱的 pH 变化研究表明，当 pH 值高于 α-氨基的 pKa 时，寡肽在水中表现出 β-折叠构象。高 pH 溶液在 215 nm 处的 CD 带强度取决于寡肽中赖氨酸残基的数量，表明高级寡肽中发色团间相互作用增加。在 D2O 中测量的红外光谱支持 pH 诱导寡肽和 e-PLL 的构象变化。
Abstract A series of monodispersed oligo(e-L-lysine)mers (2-, 4-, 6-, and 8-mers), which provides a fragment structure of microbially produced poly(e-L-lysine) (e-PLL), was synthesized by the liquid phase method. Conformations of the oligopeptides were studied using circular dichroism and infrared spectra, and compared with the results of e-PLL. The pH variation study of the CD spectra suggested that the oligopeptides exhibited a β-sheet conformation in water at pH elevated above the pKa of the α-amino group. A CD band intensity at 215 nm for the high pH solution was dependent on the number of lysine residues in the oligopeptides, indicating an increased interchromophore interaction in a higher oligopeptide. IR spectra measured in D2O supported the pH induced conformational change for the oligopeptides and e-PLL.