Calmin, a protein with calponin homology and transmembrane domains expressed in maturing spermatogenic cells
Calmin, a protein with calponin homology and transmembrane domains expressed in maturing spermatogenic cells
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DOI：
10.1006/geno.2001.6544
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发表时间：
2001-06-01
期刊：
影响因子：
4.4
通讯作者：
Huh, NH
中科院分区：
文献类型：
--
作者：
Ishisaki, Z;Takaishi, M;Huh, NH
应用RNA差异显示技术从小鼠皮肤组织中分离到一个大小约为3.2kb的calmin基因。Calmin cDNA编码1021个氨基酸，在N-末端侧具有两个串联的钙调蛋白同源（CH）结构域，在C-末端侧具有跨膜结构域。覆盖CH结构域的区域显示出与β-血影蛋白、α-辅肌动蛋白和肌营养不良蛋白的高度同源性。在具有串联CH结构域的蛋白质中，钙蛋白具有独特的跨膜结构域。在calmin的3 '端发现了三个可变剪接位点，产生了具有或不具有跨膜结构域的多态性蛋白产物。Calmin转录本在成体睾丸、肝脏、肾脏和大肠中均有表达，且在睾丸中的表达远强于其它组织。原位杂交和免疫组化结果表明，calmin在成熟后期的生精细胞中表达，并分离了人calmin cDNA，测定了其外显子/内含子结构。(C)北京：科学出版社.
A cDNA named calmin of similar to3.2 kb was isolated by RNA differential display applied to developing mouse skin. Calmin cDNA encodes 1021 amino acids with two calponin homology (CH) domains in tandem on the N-terminal side and a transmembrane domain on the C-terminal side. The region covering the CH domains showed a high level of homology with beta -spectrin, alpha -actinin, and dystrophin. Among the proteins with the tandem CH domains, calmin is unique in having a transmembrane domain. Three alternative splicing sites were identified at the 3 ' -side of calmin, giving rise to polymorphic protein products with or without the transmembrane domain. The calmin transcript was detected in adult testis, liver, kidney, and large intestine; the expression in testis was far stronger than that in the other tissues. In situ hybridization and immunostaining revealed that calmin was expressed in maturing spermatogenic cells at later stages, Human calmin cDNA was also isolated, and its exon/intron organization was determined. (C) 2001 Academic Press.