Sample Preparation on Micro-Electrode-Dot-Array Digital Microfluidic Biochips
Sample Preparation on Micro-Electrode-Dot-Array Digital Microfluidic Biochips
复制标题
微电极点阵数字微流控生物芯片的样品制备
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Chen
中科院分区：
文献类型：
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作者：
Zipeng Li;Kelvin Yi;K. Chakrabarty;Tsung;Chen
数字微流控中的样品制备是指为芯片生化应用生成具有目标浓度的液滴。近年来，数字微流控生物芯片（DMFB）已被用作样品制备平台。然而，传统 DMFB 上的样品制备仍然存在一个主要问题。对于传统的 DMFB，只能使用 (1:1) 混合/分流模型，导致样品制备所需的流体操作数量增加。为了克服这个缺点，我们采用下一代 DMFB 平台，称为微电极点阵列 (MEDA) 来进行样品制备。我们提出了第一种样品制备方法，该方法利用了 MEDA 特有的液滴尺寸细粒度控制和实时液滴传感的优势。使用制造的 MEDA 生物芯片进行的实验演示和模拟结果突出了所提出的样品制备方法的有效性。
Sample preparation in digital microfluidics refers to the generation of droplets with target concentrations for onchip biochemical applications. In recent years, digital microfluidic biochips (DMFBs) have been adopted as a platform for sample preparation. However, there remain one major problem associated with sample preparation on a conventional DMFB. For conventional DMFBs, only a (1:1) mixing/splitting model can be used, leading to an increase in the number of fluidic operations required for sample preparation. To overcome the drawback, we adopt a next generation DMFB platform, referred to as micro-electrode-dot-array (MEDA), for sample preparation. We propose the first sample preparation method that exploits the MEDA-specific advantages of fine-grained control of droplet sizes and real-time droplet sensing. Experimental demonstration using a fabricated MEDA biochip and simulation results highlight the effectiveness of the proposed sample-preparation method.